Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab103203 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
ICC/IF Use at an assay dependent concentration.

Target

  • RelevanceDMP1 is an extracellular matrix protein and a member of the small integrin binding ligand N-linked glycoprotein family. It is critical for proper mineralization of bone and dentin, and is present in diverse cells of bone and tooth tissues. DMP1 contains a large number of acidic domains, multiple phosphorylation sites, a functional arg-gly-asp cell attachment sequence, and a DNA binding domain. DMP1 may also have a dual function during osteoblast differentiation. In the nucleus of undifferentiated osteoblasts the unphosphorylated form acts as a transcriptional component for activation of osteoblast-specific genes like osteocalcin. During the osteoblast to osteocyte transition phase it is phosphorylated and exported into the extracellular matrix, where it regulates nucleation of hydroxyapatite.
  • Cellular localizationNucleus. Cytoplasm. Secreted; extracellular space; extracellular matrix. Note=In proliferating preosteoblasts it is nuclear, during early maturation stage is cytoplasmic and in mature osteoblast localizes in the mineralizated matrix. Export from the nucleus of differentiating osteoblast is triggered by the release of calcium from intracellular stores followed by a massive influx of this pool of calcium into the nucleus.
  • Database links
  • Alternative names
    • ARHP antibody
    • ARHR antibody
    • AV020965 antibody
    • Dentin matrix acidic phosphoprotein 1 antibody
    • Dentin matrix protein 1 antibody
    • DENTMAT antibody
    • DMP 1 antibody
    • Dmp antibody
    • MGC130441 antibody
    • PP antibody
    • Serine rich acidic phosphoprotein antibody
    see all

Anti-DMP1 antibody images

  • Anti-DMP1 antibody (ab103203) at 1 µg/ml + Bone Marrow (Human) Tissue Lysate - adult normal tissue at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 55 kDa
    Observed band size : 55 kDa
    Additional bands at : 50 kDa (possible truncated form).

    Exposure time : 4 minutes
  • Anti-DMP1 antibody (ab103203) at 1/200 dilution + whole cell lysate prepared from murine bone marrow stromal osteoblasts at 20000 cells

    Secondary
    HRP-conjugated goat polyclonal at 1/120000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 55 kDa
    Observed band size : 55 kDa


    Exposure time : 30 seconds

    Image courtesy of an anonymous Abreview.

    See Abreview

References for Anti-DMP1 antibody (ab103203)

This product has been referenced in:
  • Lee HK  et al. Nuclear factor I-C (NFIC) regulates dentin sialophosphoprotein (DSPP) and E-cadherin via control of Krüppel-like factor 4 (KLF4) during dentinogenesis. J Biol Chem 289:28225-36 (2014). Read more (PubMed: 25138274) »
  • Martin A  et al. Overexpression of the DMP1 C-terminal fragment stimulates FGF23 and exacerbates the hypophosphatemic rickets phenotype in Hyp mice. Mol Endocrinol 26:1883-95 (2012). IHC-P ; Mouse . Read more (PubMed: 22930691) »

See all 2 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Antigen retrieval step Enzymatic - Buffer/Enzyme Used: Proteinase K
Sample Mouse Tissue sections (Tibia)
Specification Tibia
Permeabilization Yes - Methanol
Fixative Paraformaldehyde
Username

Mr. Vasily Gnyubkin

Verified customer

Submitted Sep 11 2013

Application Western blot
Sample Human Cell lysate - whole cell (PULP)
Gel Running Conditions Non-reduced Non-Denaturing (Native)
Loading amount 40 µg
Specification PULP
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Ms. kyungjoo seong

Verified customer

Submitted Nov 30 2016

Application Western blot
Sample Mouse Tissue lysate - whole (Bone Tissue)
Gel Running Conditions Reduced Denaturing (12)
Loading amount 40 µg
Treatment 100 ug/ml of Insulin
Specification Bone Tissue
Blocking step Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
Username

Dr. FRANCESCO ELIA MARINO

Verified customer

Submitted Oct 16 2015

Thank you for taking the time to complete our questionnaire. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody. The details you have kindly provided will enable us to investigate this case for you and this is ...

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Thank you for contacting us.

I am sorry to hear that you are experiencing difficulties with this product ab103203 in western blot. Often it is possible to make suggestions that help resolve problems. We will happily offer technical support an...

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Thank you for contacting us.
We have many antibodies that specifically stain endothelial and osteoblast marker proteins. The most popular antibodies are as follows;
Osteocalcin, DMP-1 and Osteopontin are Osteoblast marker proteins, the antibo...

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Application Immunocytochemistry/ Immunofluorescence
Sample Sheep Cell (Ovine fibroblasts from skin biopsies)
Specification Ovine fibroblasts from skin biopsies
Fixative Paraformaldehyde
Permeabilization No
Username

Abcam user community

Verified customer

Submitted May 19 2011

Application Western blot
Sample Mouse Cell lysate - whole cell (Bone marrow stromal cells/osteoblasts)
Loading amount 20000 cells
Specification Bone marrow stromal cells/osteoblasts
Treatment 50microg/ml L-ascorbic acid, 10 mM betha-glycerophosphate for 7days
Gel Running Conditions Reduced Denaturing (4-10%)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Mar 23 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"