Overview

  • Product nameAnti-DMP1 antibody
    See all DMP1 primary antibodies
  • Description
    Mouse monoclonal to DMP1
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human, Recombinant Fragment
  • Immunogen

    Recombinant fragment, corresponding to amino acids 221-331 of Human DMP1 (NP_004398) with tag.

  • Positive control
    • Recombinant tagged human DMP1 fragment.

Properties

Applications

Our Abpromise guarantee covers the use of ab76632 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesELISA: Use at an assay dependent dilution.
    WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 56 kDa.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceDMP1 is an extracellular matrix protein and a member of the small integrin binding ligand N-linked glycoprotein family. It is critical for proper mineralization of bone and dentin, and is present in diverse cells of bone and tooth tissues. DMP1 contains a large number of acidic domains, multiple phosphorylation sites, a functional arg-gly-asp cell attachment sequence, and a DNA binding domain. DMP1 may also have a dual function during osteoblast differentiation. In the nucleus of undifferentiated osteoblasts the unphosphorylated form acts as a transcriptional component for activation of osteoblast-specific genes like osteocalcin. During the osteoblast to osteocyte transition phase it is phosphorylated and exported into the extracellular matrix, where it regulates nucleation of hydroxyapatite.
    • Cellular localizationNucleus. Cytoplasm. Secreted; extracellular space; extracellular matrix. Note=In proliferating preosteoblasts it is nuclear, during early maturation stage is cytoplasmic and in mature osteoblast localizes in the mineralizated matrix. Export from the nucleus of differentiating osteoblast is triggered by the release of calcium from intracellular stores followed by a massive influx of this pool of calcium into the nucleus.
    • Database links
    • Alternative names
      • ARHP antibody
      • ARHR antibody
      • AV020965 antibody
      • Dentin matrix acidic phosphoprotein 1 antibody
      • Dentin matrix protein 1 antibody
      • DENTMAT antibody
      • DMP 1 antibody
      • Dmp antibody
      • MGC130441 antibody
      • PP antibody
      • Serine rich acidic phosphoprotein antibody
      see all

    Anti-DMP1 antibody images

    • Anti-DMP1 antibody (ab76632) at 1 µg/ml + Recombinant tagged human DMP1 fragment at 0.2 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

      Predicted band size : 56 kDa
      Observed band size : 38 kDa (why is the actual band size different from the predicted?)

    References for Anti-DMP1 antibody (ab76632)

    This product has been referenced in:
    • Pilia M  et al. Influence of substrate curvature on osteoblast orientation and extracellular matrix deposition. J Biol Eng 7:23 (2013). Human . Read more (PubMed: 24090183) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"