Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab82351 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250. Detects a band of approximately 50 kDa (predicted molecular weight: 55 kDa).
IHC-P 1/100.

Target

  • RelevanceDMP1 is an extracellular matrix protein and a member of the small integrin binding ligand N-linked glycoprotein family. It is critical for proper mineralization of bone and dentin, and is present in diverse cells of bone and tooth tissues. DMP1 contains a large number of acidic domains, multiple phosphorylation sites, a functional arg-gly-asp cell attachment sequence, and a DNA binding domain. DMP1 may also have a dual function during osteoblast differentiation. In the nucleus of undifferentiated osteoblasts the unphosphorylated form acts as a transcriptional component for activation of osteoblast-specific genes like osteocalcin. During the osteoblast to osteocyte transition phase it is phosphorylated and exported into the extracellular matrix, where it regulates nucleation of hydroxyapatite.
  • Cellular localizationNucleus. Cytoplasm. Secreted; extracellular space; extracellular matrix. Note=In proliferating preosteoblasts it is nuclear, during early maturation stage is cytoplasmic and in mature osteoblast localizes in the mineralizated matrix. Export from the nucleus of differentiating osteoblast is triggered by the release of calcium from intracellular stores followed by a massive influx of this pool of calcium into the nucleus.
  • Database links
  • Alternative names
    • ARHP antibody
    • ARHR antibody
    • AV020965 antibody
    • Dentin matrix acidic phosphoprotein 1 antibody
    • Dentin matrix protein 1 antibody
    • DENTMAT antibody
    • DMP 1 antibody
    • Dmp antibody
    • MGC130441 antibody
    • PP antibody
    • Serine rich acidic phosphoprotein antibody
    see all

Anti-DMP1 antibody images

  • ab82351 staining DMP1 in human tooth tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with methanol and blocked with 5% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/100) for 24 hours at 4°C. An undiluted HRP-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Anti-DMP1 antibody (ab82351) at 1/250 dilution + Bone Marrow (Human) Tissue Lysate - adult normal tissue at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 55 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)


    Exposure time : 8 minutes
    The band observed at 50 kDa could potentially be a cleaved form of DMP1 due to the presence of a 16 amino acid signal peptide.

References for Anti-DMP1 antibody (ab82351)

This product has been referenced in:
  • Lee HS  et al. Comparative gene-expression analysis of the dental follicle and periodontal ligament in humans. PLoS One 8:e84201 (2013). IHC-P ; Human . Read more (PubMed: 24376796) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step None
Sample Human Tissue sections (Follicle)
Specification Follicle
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Apr 08 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step None
Sample Human Tissue sections (tooth)
Specification tooth
Permeabilization Yes - Methanol
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Mar 18 2014

DISCOUNT CODE: XXXXXXX
Expiration date: 20-Nov.-2012

I am very pleased to hear you would like to accept our offer and test ab82351 in IHC-P. This code will give you: 1 free [PRIMARY ANTIBODY/PROTEIN] OR VALUE OFF OF ORDER before the expira...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"