Anti-DMPO Nitrone Adduct antibody (ab23702)
Key features and details
- Rabbit polyclonal to DMPO Nitrone Adduct
- Suitable for: WB, ELISA
- Reacts with: Species independent
- Isotype: IgG
Overview
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Product name
Anti-DMPO Nitrone Adduct antibody
See all DMPO Nitrone Adduct primary antibodies -
Description
Rabbit polyclonal to DMPO Nitrone Adduct -
Host species
Rabbit -
Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
5,5-dimethyl-2-(8-octanoic acid)-1-pyrroline-N-oxide conjugated to Ovalbumin.
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General notes
Measurement of protein radicals is now possible with the development of the "spin trap immunoassay" (please see refs 1,2). Compared to traditional techniques, immunodetection of spin trap products allows a higher level of sensitivity and throughput with greatly reduced sample consumption.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Constituent: Whole serum -
Concentration information loading...
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Purity
Whole antiserum -
Primary antibody notes
Measurement of protein radicals is now possible with the development of the "spin trap immunoassay" (please see refs 1,2). Compared to traditional techniques, immunodetection of spin trap products allows a higher level of sensitivity and throughput with greatly reduced sample consumption. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab23702 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Detects a band of approximately 17 kDa.
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ELISA |
1/1000 - 1/5000.
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Notes |
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WB
1/1000 - 1/5000. Detects a band of approximately 17 kDa. |
ELISA
1/1000 - 1/5000. |
Target
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Relevance
For measurement of protein radicals; some proteins with endogenous peroxidase activity are especially susceptible to forming DMPO adducts. -
Alternative names
- 55 dimethyl 1 pyrroline N oxide nitrone adduct antibody
Images
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All lanes : Anti-DMPO Nitrone Adduct antibody (ab23702)
Lane 1 : Metmyoglobin at 3 µl
Lane 2 : Metmyoglobin-DMPO at 3 µl
Lane 3 : Metmyoglobin-DMPO at 5 µl
Lane 4 : Metmyoglobin-DMPO at 10 µl
Observed band size: 17 kDa why is the actual band size different from the predicted?
ab23702 was used at a concentration of 1/1000 or 1/5000, as noted at the top of the image.
Datasheets and documents
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Datasheet download
References (6)
ab23702 has been referenced in 6 publications.
- Proniewski B et al. Immuno-Spin Trapping-Based Detection of Oxidative Modifications in Cardiomyocytes and Coronary Endothelium in the Progression of Heart Failure in Tgaq*44 Mice. Front Immunol 9:938 (2018). PubMed: 29867936
- Pathak V et al. Formation of singlet oxygen by decomposition of protein hydroperoxide in photosystem II. PLoS One 12:e0181732 (2017). WB ; Spinacia oleracea . PubMed: 28732060
- Kale R et al. Amino acid oxidation of the D1 and D2 proteins by oxygen radicals during photoinhibition of Photosystem II. Proc Natl Acad Sci U S A 114:2988-2993 (2017). WB . PubMed: 28265052
- Gomez-Mejiba SE et al. Immuno-spin trapping of protein and DNA radicals: "tagging" free radicals to locate and understand the redox process. Free Radic Biol Med 46:853-65 (2009). ELISA . PubMed: 19159679
- Schildknecht S et al. Acetaminophen inhibits prostanoid synthesis by scavenging the PGHS-activator peroxynitrite. FASEB J 22:215-24 (2008). WB . PubMed: 17724253
- Ramirez DC et al. Immuno-spin trapping analyses of DNA radicals. Nat Protoc 2:512-22 (2007). PubMed: 17406615