Overview

  • Product nameAnti-DMPO Nitrone Adduct antibody
    See all DMPO Nitrone Adduct primary antibodies
  • Description
    Rabbit polyclonal to DMPO Nitrone Adduct
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Immunogen

    5,5-dimethyl-2-(8-octanoic acid)-1-pyrroline-N-oxide conjugated to Ovalbumin.

  • General notes


    Measurement of protein radicals is now possible with the development of the "spin trap immunoassay" (please see refs 1,2). Compared to traditional techniques, immunodetection of spin trap products allows a higher level of sensitivity and throughput with greatly reduced sample consumption.

Properties

Applications

Our Abpromise guarantee covers the use of ab23702 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 17 kDa.
ELISA 1/1000 - 1/5000.

Target

  • RelevanceFor measurement of protein radicals; some proteins with endogenous peroxidase activity are especially susceptible to forming DMPO adducts.
  • Alternative names
    • 55 dimethyl 1 pyrroline N oxide nitrone adduct antibody

Anti-DMPO Nitrone Adduct antibody images

  • All lanes : Anti-DMPO Nitrone Adduct antibody (ab23702)

    Lane 1 : Metmyoglobin at 3 µl
    Lane 2 : Metmyoglobin-DMPO at 3 µl
    Lane 3 : Metmyoglobin-DMPO at 5 µl
    Lane 4 : Metmyoglobin-DMPO at 10 µl


    Observed band size : 17 kDa (why is the actual band size different from the predicted?)

References for Anti-DMPO Nitrone Adduct antibody (ab23702)

This product has been referenced in:
  • Gomez-Mejiba SE  et al. Immuno-spin trapping of protein and DNA radicals: "tagging" free radicals to locate and understand the redox process. Free Radic Biol Med 46:853-65 (2009). ELISA . Read more (PubMed: 19159679) »
  • Schildknecht S  et al. Acetaminophen inhibits prostanoid synthesis by scavenging the PGHS-activator peroxynitrite. FASEB J 22:215-24 (2008). WB . Read more (PubMed: 17724253) »

See all 3 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab23702.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"