Anti-DMPO Nitrone Adduct antibody [N1664A] (ab104902)

Overview

  • Product nameAnti-DMPO Nitrone Adduct antibody [N1664A]
    See all DMPO Nitrone Adduct primary antibodies
  • Description
    Mouse monoclonal [N1664A] to DMPO Nitrone Adduct
  • SpecificityDetects ~90kDa. Recognizes DMPO, DMPO-octanoic acid, DMPO-protein adducts and DMPO-DNA adducts. Does not cross-react with non-adducted proteins or DNA.
  • Tested applicationsSuitable for: WB, ELISA, ICC/IF, IPmore details
  • Immunogen

    5,5-dimethyl-2-(8-octanoic acid)-1-pyrrolone-N-oxide conjugated to Ovalbumin.

Properties

Applications

Our Abpromise guarantee covers the use of ab104902 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 10 µg/ml.
ELISA Use a concentration of 1 - 10 µg/ml.
ICC/IF Use a concentration of 1 - 10 µg/ml.
IP Use at an assay dependent dilution. Follow the method described by Chatterjee, S. et. al. PubMed 19049863.

Target

  • RelevanceFor measurement of protein radicals; some proteins with endogenous peroxidase activity are especially susceptible to forming DMPO adducts.
  • Alternative names
    • 55 dimethyl 1 pyrroline N oxide nitrone adduct antibody

Anti-DMPO Nitrone Adduct antibody [N1664A] images

  • Immuncytochemistry/Immunofluorescence analysis of Mouse macrophage cells labelling DMPO Nitrone Adduct with ab104902 at 10μg/mL. Left - with DMPO, Right - no DMPO. Top to bottom - DMPO, DAPI, overlay.

  • All lanes : Anti-DMPO Nitrone Adduct antibody [N1664A] (ab104902) at 10 µg/ml

    Lane 1 : 10µM Hb
    Lane 2 : 10µM Hb + 100µM HOCL
    Lane 3 : 10µM Hb + 500µM HOCL
    Lane 4 : 10µM Hb + 1000µM HOCL
    Lane 5 : 10µM Hb with 20mM DMPO
    Lane 6 : 10µM Hb + 100µM HOCL with 20mM DMPO
    Lane 7 : 10µM Hb + 500µM HOCL with 20mM DMPO
    Lane 8 : 10µM Hb + 1000µM HOCL with 20mM DMPO
    Lane 9 : 10µM Hb with 100mM DMPO
    Lane 10 : 10µM Hb + 100µM HOCL with 100mM DMPO
    Lane 11 : 10µM Hb + 500µM HOCL with 100mM DMPO
    Lane 12 : 10µM Hb + 1000µM HOCL with 100mM DMPO

References for Anti-DMPO Nitrone Adduct antibody [N1664A] (ab104902)

ab104902 has not yet been referenced specifically in any publications.

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Unfortunately we do not design custom made antibodies. This particular one does not have a fluorescent molecule attached to it and requires a secondary for detection.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"