Anti-DMPO Nitrone Adduct antibody [N1664A] (Biotin) (ab110430)

Overview

  • Product name
    Anti-DMPO Nitrone Adduct antibody [N1664A] (Biotin)
    See all DMPO Nitrone Adduct primary antibodies
  • Description
    Mouse monoclonal [N1664A] to DMPO Nitrone Adduct (Biotin)
  • Conjugation
    Biotin
  • Specificity
    Recognizes DMPO, DMPO-octanoic acid, DMPO-protein adducts and DMPO-DNA adducts. Does not cross-react with non-adducted proteins or DNA.
  • Tested applications
    Suitable for: IP, ICC/IF, IHC-P, WBmore details
  • Immunogen

    5,5-dimethyl-2-(8-octanoic acid)-1-pyrrolone-N-oxide conjugated to Ovalbumin.

Properties

Applications

Our Abpromise guarantee covers the use of ab110430 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.
IHC-P Use at an assay dependent dilution.
WB Use at an assay dependent dilution.

Target

  • Relevance
    For measurement of protein radicals; some proteins with endogenous peroxidase activity are especially susceptible to forming DMPO adducts.
  • Alternative names
    • 55 dimethyl 1 pyrroline N oxide nitrone adduct antibody

Images

  • Immunofluorescent analysis of DMPO:Biotin in Mouse macrophage cell lines using ab110430 at 10ug/mL.
  • Western blot detection of DMPO using ab110430.

References

This product has been referenced in:
  • Hamasaki T  et al. Electrochemically reduced water exerts superior reactive oxygen species scavenging activity in HT1080 cells than the equivalent level of hydrogen-dissolved water. PLoS One 12:e0171192 (2017). Read more (PubMed: 28182635) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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