The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
ELISA titre using peptide based assay, 1:12500.
FunctionMay be required for testis development.
Tissue specificityTestis specific.
Sequence similaritiesBelongs to the DMRT family. Contains 1 DM DNA-binding domain.
Developmental stageExpressed in the genital ridges of six-week-old male embryos. Becomes confined to the forming seminiferous tubules (possibly Sertoli cells) at seven weeks. Not detected in female embryos.