Overview

  • Product nameAnti-DMRT3 antibody
    See all DMRT3 primary antibodies
  • Description
    Rabbit polyclonal to DMRT3
  • Tested applicationsSuitable for: WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Human, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within C terminal amino acids 438-467 of Human DMRT3 (NP_067063.1), conjugated to KLH.

  • Positive control
    • Mouse kidney tissue lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab107043 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 51 kDa.
IHC-Fr 1/100.

Target

  • FunctionMay regulate transcription during sexual development.
  • Tissue specificitySpecifically expressed in testis.
  • Sequence similaritiesBelongs to the DMRT family.
    Contains 1 DM DNA-binding domain.
  • Developmental stageExpressed in 4 to 5 weeks embryos.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DMRT 3 antibody
    • DMRT like family A3 antibody
    • dmrt3 antibody
    • DMRT3_HUMAN antibody
    • DMRTA3 antibody
    • Doublesex and mab 3 related transcription factor 3 antibody
    • Doublesex- and mab-3-related transcription factor 3 antibody
    • MGC142144 antibody
    • Testis specific protein antibody
    see all

Anti-DMRT3 antibody images

  • IHC-Fr image of DMRT3 staining on zebrafish retina sections using ab107043 (1:100). The sections were paraformaldehyde and permeabilized using Triton-X. Antigen retrieval was performed using Tris-HCl and blocking was perfomed using 5% BSA for 1 hour at 23°C. ab107043 was diluted 1:100 and incubated with the sections for 16 hours at 4°C. The secondary antibody was goat polyclonal to rabbit IgG conjugated to Alexa Fluor 488 (1:1000).

    See Abreview

  • Anti-DMRT3 antibody (ab107043) at 1/100 dilution + Mouse kidney tissue lysate at 35 µg

    Predicted band size : 51 kDa

References for Anti-DMRT3 antibody (ab107043)

ab107043 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Sample Zebrafish Tissue sections (Retinal Neurons, forebrain neurons)
Specification Retinal Neurons, forebrain neurons
Permeabilization Yes - triton X
Fixative Paraformaldehyde
Username

Dr. Ryan MacDonald

Verified customer

Submitted Aug 22 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"