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Recombinant Human DMT1 protein (NP_000608.1).
Our Abpromise guarantee covers the use of ab117544 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 62 kDa.|
|IHC-P||Use a concentration of 5 µg/ml.|
|Sandwich ELISA||Use at an assay dependent concentration.|
ICC/IF image of DMT1 staining on Caco2 cells using ab117544 (1:1000). The cells were fixed with paraformaldehyde and permeabilized using 0.1% TritonX in 0.1% PBS. The cells were blocked using 10 % donkey serum for 1 hour at 24°C. ab117544 was diluted 1:1000 using 0.1% TritonX with 0.1x PBS- 10% Donkeys and incubated with the slides at 4°C for 24 hours. The secondary antibody used was donkey polyclonal against mouse IgG conjugated to Alexa Fluor 488 (undiluted)
ab117544 has not yet been referenced specifically in any publications.