Anti-DMT1 antibody (ab140977)
Key features and details
- Rabbit polyclonal to DMT1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DMT1 antibody
See all DMT1 primary antibodies -
Description
Rabbit polyclonal to DMT1 -
Host species
Rabbit -
Specificity
BLAST analysis of the peptide immunogen showed no homology with other Human proteins. -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Horse, Hamster, Cow, Chimpanzee, Monkey, Gorilla, Common marmoset, Orangutan, Elephant -
Immunogen
Synthetic 16 amino acid peptide from the internal region of Human DMT1.
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Positive control
- Human pancreas and kidney tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. -
Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab140977 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (2) |
Use a concentration of 15 - 20 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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IHC-P
Use a concentration of 15 - 20 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Important in metal transport, in particular iron. Can also transport manganese, cobalt, cadmium, nickel, vanadium and lead. Involved in apical iron uptake into duodenal enterocytes. Involved in iron transport from acidified endosomes into the cytoplasm of erythroid precursor cells. May play an important role in hepatic iron accumulation and tissue iron distribution. -
Tissue specificity
Ubiquitously expressed. Isoform 1 is highly expressed in brain. Isoform 2 is highly expressed in spleen, thymus and pancreas. Isoform 3 and isoform 4 are abundantly expressed in duodenum and kidney. -
Involvement in disease
Defects in SLC11A2 are a cause of hypochromic microcytic anemia (HCMA) [MIM:206100]. The disease is characterized by an abnormal hemoglobin content in the erythrocytes which are reduced in size. It may be hereditary or acquired. Mutations in SLC11A2 are associated with progressive liver iron overload and normal to moderately elevated serum ferritin levels. -
Sequence similarities
Belongs to the NRAMP family. -
Post-translational
modificationsUbiquitinated by WWP2. -
Cellular localization
Endosome membrane. - Information by UniProt
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Database links
- Entrez Gene: 451897 Chimpanzee
- Entrez Gene: 100399071 Common marmoset
- Entrez Gene: 521189 Cow
- Entrez Gene: 4891 Human
- Entrez Gene: 18174 Mouse
- Entrez Gene: 25715 Rat
- Omim: 600523 Human
- SwissProt: P49281 Human
see all -
Alternative names
- DCT 1 antibody
- dct-1 antibody
- DCT1 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab140977 has been referenced in 2 publications.
- van de Looij Y et al. Nutritional Intervention for Developmental Brain Damage: Effects of Lactoferrin Supplementation in Hypocaloric Induced Intrauterine Growth Restriction Rat Pups. Front Endocrinol (Lausanne) 10:46 (2019). PubMed: 30800096
- Ito H et al. Cancer cell-specific mitochondrial reactive oxygen species promote non-heme iron uptake and enhance the proliferation of gastric epithelial cancer cell. J Clin Biochem Nutr 61:183-188 (2017). PubMed: 29203959