Anti-DMTF1 antibody (ab177246)
Key features and details
- Goat polyclonal to DMTF1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-DMTF1 antibody
See all DMTF1 primary antibodies -
Description
Goat polyclonal to DMTF1 -
Host species
Goat -
Specificity
ab177246 is expected to recognize both reported isoforms (NP_066968.3; NP_001135798.1). Reported variants represent identical protein: NP_066968.3, NP_001135799.1. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Dog, Pig -
Immunogen
Synthetic peptide corresponding to Human DMTF1 aa 629-642 (internal sequence) (Cysteine residue).
Sequence:DAHVSKFSDQNSTE
Database link: NP_066968.3 -
Positive control
- Human, mouse and rat testis lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab177246 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab177246 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 84 kDa.
1 hour primary incubation is recommended for this product. |
Notes |
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WB
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 84 kDa. 1 hour primary incubation is recommended for this product. |
Target
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Function
Transcriptional activator which activates the CDKN2A/ARF locus in response to Ras-Raf signaling, thereby promoting p53/TP53-dependent growth arrest (By similarity). Binds to the consensus sequence 5'-CCCG[GT]ATGT-3' (By similarity). Isoform 1 may cooperate with MYB to activate transcription of the ANPEP gene. Isoform 2 may antagonize transcriptional activation by isoform 1. -
Tissue specificity
Expressed at relatively low levels in colonic mucosa, ovary, peripheral leukocytes, prostate and small intestine, and at higher levels in spleen, testis and thymus. Expressed in multiple regions of the brain and CNS including amygdala, caudate, corpus callosum, hippocampus, substantia nigra and subthalamic nucleus. Isoform 1 is the predominant isoform in monocytes, macrophages and neutrophils, isoform 2 is most strongly expressed in peripheral blood leukocytes and quiescent CD34 positive cells, and isoform 3 is expressed at low levels in all hematopoietic cell types. Expression is frequently reduced in non-small-cell lung carcinomas (NSCLC) due to hemizygous gene deletion, strongly suggesting that this locus is haploinsufficient for tumor suppression. Loss of this locus frequently occurs in tumors which retain wild-type CDKN2A/ARF and p53/TP53 loci. Hemizygous gene deletion has also been observed in leukemic blasts from patients with abnormalities of the long arm of chromosome 7. -
Sequence similarities
Belongs to the DMTF1 family.
Contains 1 HTH myb-type DNA-binding domain.
Contains 2 Myb-like domains. -
Developmental stage
Isoform 2 expression is down-regulated during myeloid differentiation, while the expression of isoform 1 and isoform 3 remain constant. -
Post-translational
modificationsPhosphorylated by the cyclin-D2/CDK4, cyclin-D3/CDK4 and cyclin-D2/CDK6 complexes and to a lesser extent by the cyclin-D1/CDK4 complex. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 539676 Cow
- Entrez Gene: 475217 Dog
- Entrez Gene: 9988 Human
- Entrez Gene: 23857 Mouse
- Entrez Gene: 100521327 Pig
- Entrez Gene: 114485 Rat
- Omim: 608491 Human
- SwissProt: Q9Y222 Human
see all -
Alternative names
- Cyclin D binding Myb like protein antibody
- Cyclin D binding Myb like transcription factor 1 antibody
- cyclin D binding myb like transcription factor 1D interacting myb like protein antibody
see all
Images
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All lanes : Anti-DMTF1 antibody (ab177246) at 1 µg/ml
Lane 1 : Human testis lysate (in RIPA buffer)
Lane 2 : Mouse testis lysate (in RIPA buffer)
Lane 3 : Rat testis lysate (in RIPA buffer)
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 84 kDaPrimary incubation was 1 hour.
Datasheets and documents
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Datasheet download
References (0)
ab177246 has not yet been referenced specifically in any publications.