DNA demethylase (total) Activity Quantification Assay Kit (ab113472)

Overview

  • Product nameDNA demethylase (total) Activity Quantification Assay Kit
    See all DNA demethylase kits
  • Assay typeEnzyme activity
  • Assay time
    3h 00m
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: all Mammals
  • Product overview

    DNA methylation plays an essential role in biological processess through repression of transcription, while DNA demethylation is necessary, on the other hand, for epigenetic reprogramming of these genes. Active demethylation of DNA requires specific demethylase participation such as from MBD2.

    DNA demethylase (total) Activity Quantification Assay Kit (ab113472) allows the user to measure total DNA demethylase activity/inhibition at tremendously fast speeds and consistency; superior and safer than all other current methods. The kit is ready-to-use and provides all the essential components needed for measuring total DNA demethylase activity/inhibition.

    ab113472 is suitable for measuring DNA demethylase activity/inhibition from cultured cells/tissues or using purified enzyme/protein extracts.

  • Notes

     

     

  • Tested applicationsSuitable for: Functional Studiesmore details

Properties

    Applications

    Our Abpromise guarantee covers the use of ab113472 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent concentration.

    DNA demethylase (total) Activity Quantification Assay Kit images

    • Nuclear extracts were prepared from MCF-7 cells using the Nuclear Extraction Kit (ab113474) and total DNA demethylase activity was measured using ab113472.

    Protocols

    References for DNA demethylase (total) Activity Quantification Assay Kit (ab113472)

    ab113472 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting us. The substrate for ab113472 is double stranded DNA. We currently only have the information related to use of this kit with DNA from mammalian tissue/cells. However, based on the working principle, this kit should also work o...

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    Thank you for contacting us.
    The kit ab113472 is designed to detect DNA demethylase activity and will detect the this activity using a unique methylated DNA substrate. The detection is then colorimetric and can be measured by optical density measu...

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    Glycerol, Triton X-100 etc might interfere with the kit components. ab113474 is the optimized kit for sample preparation.

    The detection is HRP-based and will not interfere by other enzymes/proteins.
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    Thank you for contacting us.

    The compatible kit for sample preparation is ab113474. The chemicals in this kit are selected in such a way that these do not interfere with ab113472.

    http://www.abcam.com/EpiSeeker-Nuclear-Extraction-Kit...

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    Thank you once again for your enquiries. I very much appreciate your patience waiting for a response.

    I have been in touch with the originator of the buffer and the kit. In conclusion, they have retested and have established that using the b...

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    Thank you for taking the time to contact us.Your email has been forwarded to me as I was originally dealing with this case. I am sorry to hear your colleague Ana is stillhaving difficulty obtaining satisfactory results from this kit

    I would ...

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    Thank you for your recent telephone enquiry. I am sorry to hear you have some concerns regarding the results from this kit. In answer to yourquestions: 1. We are obtaining higher readings from our control that our samples, please explain how we can cal...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"