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DNA methylation plays an essential role in biological processess through repression of transcription, while DNA demethylation is necessary, on the other hand, for epigenetic reprogramming of these genes. Active demethylation of DNA requires specific demethylase participation such as from MBD2.
DNA demethylase (total) Activity Quantification Assay Kit (ab113472) allows the user to measure total DNA demethylase activity/inhibition at tremendously fast speeds and consistency; superior and safer than all other current methods. The kit is ready-to-use and provides all the essential components needed for measuring total DNA demethylase activity/inhibition.
ab113472 is suitable for measuring DNA demethylase activity/inhibition from cultured cells/tissues or using purified enzyme/protein extracts.
|Components||48 tests||96 tests|
|10X Wash Buffer||1 x 11ml||1 x 22ml|
|8-Well Assay Strips (with Frame)||6 units||12 units|
|Capture Antibody, 1000 µg/mL||1 x 5µl||1 x 8µl|
|Demethylase Assay Buffer||1 x 3ml||1 x 6ml|
|Demethylase Substrate||1 x 0.1ml||1 x 0.2ml|
|Demethylation Standard, 10 µg/mL||1 x 20µl||1 x 40µl|
|Detecting Antibody, 200 µg/mL||1 x 10µl||1 x 20µl|
|Developing Solution||1 x 5ml||1 x 10ml|
|Stop Solution||1 x 3ml||1 x 6ml|
|Substrate Binding Solution||1 x 5ml||1 x 10ml|
Our Abpromise guarantee covers the use of ab113472 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
Nuclear extracts were prepared from MCF-7 cells using the Nuclear Extraction Kit (ab113474) and total DNA demethylase activity was measured using ab113472.
ab113472 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"