Overview

  • Product nameAnti-DNA Gyrase A antibody [4D3]
    See all DNA Gyrase A primary antibodies
  • Description
    Mouse monoclonal [4D3] to DNA Gyrase A
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Escherichia coli
  • Immunogen

    Purified E. coli DNA Gyrase A protein (over expressed from E. coli)

  • Positive control
    • E. coli cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.01% None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notesPurified from tissue culture supernatant on a Protein A column using the low salt method. The antibody is purified to >95% purity as judged by SDS polyacrylamide gel electrophoresis.
  • ClonalityMonoclonal
  • Clone number4D3
  • IsotypeIgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab75585 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: 1/1000.
    Can be used in Western Blots to detect purified over expressed Gyrase A and unexpressed Gyrase in cell extracts.
    Primary antibody can be diluted 1/1000 for detection of purified proteins and 1/200 for cell extracts.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceDNA Gyrase A belongs to the topoisomerase gyrA/parC subunit family. DNA gyrase negatively supercoils closed circular double stranded DNA in an ATP dependent manner and also catalyzes the interconversion of other topological isomers of double stranded DNA rings, including catenanes and knotted rings. It is made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer. When the enzyme transiently cleaves DNA a phosphotyrosine bond is formed between the gyrA and DNA.
    • Database links
      • Alternative names
        • DNA gyrase subunit A antibody
        • GyrA antibody
        • HisW antibody
        • NalA antibody
        • ParD antibody
        see all

      Anti-DNA Gyrase A antibody [4D3] images

      • All lanes : Anti-DNA Gyrase A antibody [4D3] (ab75585) at 1/1000 dilution

        Lane 1 : B24 (a domain of the DNA Gyrase B protein)
        Lane 2 : B43 (a domain of the DNA Gyrase B protein)
        Lane 3 : B (the whole DNA Gyrase B protein)
        Lane 4 : A59 (a domain of the DNA Gyrase A protein)
        Lane 5 : A (the whole DNA Gyrase A protein)

        Lysates/proteins at 200 µg per lane.

        Secondary
        HRP labelled rabbit anti mouse IgG at 1/5000 dilution

        Observed band size : 97 kDa (why is the actual band size different from the predicted?)

      References for Anti-DNA Gyrase A antibody [4D3] (ab75585)

      ab75585 has not yet been referenced specifically in any publications.

      Product Wall

      There are currently no Abreviews or Questions for ab75585.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"