Anti-DNA Gyrase A antibody [7F11] (ab75592)


  • Product nameAnti-DNA Gyrase A antibody [7F11]
    See all DNA Gyrase A primary antibodies
  • Description
    Mouse monoclonal [7F11] to DNA Gyrase A
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Escherichia coli
  • Immunogen

    Full length purified E.coli DNA Gyrase A protein (over-expressed from E.coli)

  • EpitopeN-terminal domain of DNA Gyrase A.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notesab75592 is purified on a Protein A column using the low-salt method. It is purified to >95% purity as judged by SDS-polyacrylamide gel electrophoresis.
  • ClonalityMonoclonal
  • Clone number7F11
  • IsotypeIgG2b
  • Research areas


Our Abpromise guarantee covers the use of ab75592 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesWB: 1/200 - 1/1000. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).
    Can be used in Western Blots to detect purified over-expressed DNA Gyrase A and DNA Gyrase A in cell extracts.
    Primary antibody is diluted 1/1000 for detection of purified proteins and 1/200 for cell extracts.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceDNA Gyrase A belongs to the topoisomerase gyrA/parC subunit family. DNA gyrase negatively supercoils closed circular double stranded DNA in an ATP dependent manner and also catalyzes the interconversion of other topological isomers of double stranded DNA rings, including catenanes and knotted rings. It is made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer. When the enzyme transiently cleaves DNA a phosphotyrosine bond is formed between the gyrA and DNA.
    • Database links
      • Alternative names
        • DNA gyrase subunit A antibody
        • GyrA antibody
        • HisW antibody
        • NalA antibody
        • ParD antibody
        see all

      Anti-DNA Gyrase A antibody [7F11] images

      • All lanes : Anti-DNA Gyrase A antibody [7F11] (ab75592) at 1/1000 dilution

        Lane 1 : Gyrase B, B24 domain
        Lane 2 : Gyrase B, B43 domain
        Lane 3 : Gyrase B, whole protein
        Lane 4 : Gyrase A, A59 domain
        Lane 5 : Gyrase A, whole protein

        HRP labelled rabbit anti-mouse IgGs at 1/5000 dilution

        Predicted band size : 97 kDa
        Observed band size : 97 kDa

      References for Anti-DNA Gyrase A antibody [7F11] (ab75592)

      ab75592 has not yet been referenced specifically in any publications.

      Product Wall

      Thank you for your enquiry. ab75592 and ab75593 are monclonal antibodies specific to different E coli gyrase protein domains so I'm not sure whether they will react. We haven't tested them against S. aureus gyrase. We have two polyclonal antibodies ...

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