• Product nameAnti-DNA Gyrase A antibody
    See all DNA Gyrase A primary antibodies
  • Description
    Rabbit polyclonal to DNA Gyrase A
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Escherichia coli , Staphylococcus aureus
  • Immunogen

    Purified E. coli DNA Gyrase A protein (over expressed from E. coli)

  • Positive control
    • E.coli cell lysate


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notesThe antibody is isolated from rabbit blood and further purified on a Protein A column using the low salt method.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab75594 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesWB: 1/1000.
    Can be used in Western Blots to detect purified over expressed Gyrase A and unexpressed gyrase in cell extracts.
    The antibody is diluted 1/1000 for detection of purified proteins and 1/200 for cell extracts.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceDNA Gyrase A belongs to the topoisomerase gyrA/parC subunit family. DNA gyrase negatively supercoils closed circular double stranded DNA in an ATP dependent manner and also catalyzes the interconversion of other topological isomers of double stranded DNA rings, including catenanes and knotted rings. It is made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer. When the enzyme transiently cleaves DNA a phosphotyrosine bond is formed between the gyrA and DNA.
    • Database links
      • Alternative names
        • DNA gyrase subunit A antibody
        • GyrA antibody
        • HisW antibody
        • NalA antibody
        • ParD antibody
        see all

      Anti-DNA Gyrase A antibody images

      • All lanes : Anti-DNA Gyrase A antibody (ab75594) at 1/1000 dilution

        Lane 1 : E coli cell lysate (Gyrase A, A59 domain) at 0.1 µg
        Lane 2 : E coli cell lysate (Gyrase B, B43 domain) at 0.1 µg
        Lane 3 : E coli cell lysate (Gyrase B, whole protein) at 1 µg
        Lane 4 : E coli cell lysate (Gyrase B, whole protein) at 0.5 µg
        Lane 5 : E coli cell lysate (Gyrase B, whole protein) at 0.1 µg
        Lane 6 : E coli cell lysate (Gyrase A, whole protein) at 1 µg
        Lane 7 : E coli cell lysate (Gyrase A, whole protein) at 0.5 µg
        Lane 8 : E coli cell lysate (Gyrase A, whole protein) at 0.1 µg

        goat anti rabbit Igs at 1/5000 dilution

        Observed band size : 97 kDa (why is the actual band size different from the predicted?)

      References for Anti-DNA Gyrase A antibody (ab75594)

      This product has been referenced in:
      • Aedo S & Tse-Dinh YC Isolation and quantitation of topoisomerase complexes accumulated on escherichia coli chromosomal DNA. Antimicrob Agents Chemother 56:5458-64 (2012). WB . Read more (PubMed: 22869559) »

      See 1 Publication for this product

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      Most of our antibodies are stable at -20C for up to five years. For short term storage we recommend storing at 4C. It is also important to aliquot them in order to minimize damage due to freezing and thawing.
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