Anti-DNA Gyrase B antibody [9G8] (ab75593)

Overview

  • Product nameAnti-DNA Gyrase B antibody [9G8]
    See all DNA Gyrase B primary antibodies
  • Description
    Mouse monoclonal [9G8] to DNA Gyrase B
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Escherichia coli
  • Immunogen

    Purified Escherichia coli DNA Gyrase B protein (over expressed from Escherichia coli)

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notesProduced from tissue culture supernatant and purified on a Protein A column using the low salt method.
  • ClonalityMonoclonal
  • Clone number9G8
  • IsotypeIgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab75593 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: 1/1000.
    Can be used in Western Blots to detect purified over expressed Gyrase B and unexpressed gyrase in cell extracts.
    The antibody can be diluted 1/1000 for detection of purified proteins and 1/200 for cell extracts.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceDNA Gyrase B belongs to the type II topoisomerase family. DNA gyrase negatively supercoils closed circular double stranded DNA in an ATP dependent manner and also catalyzes the interconversion of other topological isomers of double stranded DNA rings, including catenanes and knotted rings. It is made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.
    • Database links
      • Alternative names
        • acrB antibody
        • DNA gyrase subunit B antibody
        • himB antibody
        • hisU antibody
        • nalC antibody
        • parA antibody
        • pcbA antibody
        see all

      Anti-DNA Gyrase B antibody [9G8] images

      • All lanes : Anti-DNA Gyrase B antibody [9G8] (ab75593) at 1/1000 dilution

        Lane 1 : B24 domain of Gyrase B protein (purified from E.coli)
        Lane 2 : B43 domain of Gyrase B protein (purified from E.coli)
        Lane 3 : Gyrase B whole protein (purified from E.coli)
        Lane 4 : A59 domain of Gyrase A protein (purified from E.coli)
        Lane 5 : Gyrase A whole protein (purified from E.coli)

        Lysates/proteins at 200 µg per lane.

        Secondary
        HRP labelled rabbit anti mouse IgGs at 1/5000 dilution

        Observed band size : 90 kDa (why is the actual band size different from the predicted?)

      References for Anti-DNA Gyrase B antibody [9G8] (ab75593)

      ab75593 has not yet been referenced specifically in any publications.

      Product Wall

      Thank you for your enquiry. ab75592 and ab75593 are monclonal antibodies specific to different E coli gyrase protein domains so I'm not sure whether they will react. We haven't tested them against S. aureus gyrase. We have two polyclonal antibodies ...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"