Anti-DNA Gyrase B antibody (ab75596)

Overview

  • Product nameAnti-DNA Gyrase B antibody
    See all DNA Gyrase B primary antibodies
  • Description
    Rabbit polyclonal to DNA Gyrase B
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Escherichia coli , Staphylococcus aureus
  • Immunogen

    Purified E. coli Gyr B protein (over expressed from E. coli)

  • Positive control
    • E. coli cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notesThis antibody is isolated from rabbit blood and further purified on a Protein A column using the low salt method.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab75596 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000.

Can be used in Western Blots to detect purified over expressed Gyrase B and unexpressed gyrase in cell extracts. The antibody can be diluted 1/1000 for detection of purified proteins and 1/200 for cell extracts.

Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Target

  • RelevanceDNA Gyrase B belongs to the type II topoisomerase family. DNA gyrase negatively supercoils closed circular double stranded DNA in an ATP dependent manner and also catalyzes the interconversion of other topological isomers of double stranded DNA rings, including catenanes and knotted rings. It is made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.
  • Database links
    • Alternative names
      • acrB antibody
      • DNA gyrase subunit B antibody
      • himB antibody
      • hisU antibody
      • nalC antibody
      • parA antibody
      • pcbA antibody
      see all

    Anti-DNA Gyrase B antibody images

    • All lanes : Anti-DNA Gyrase B antibody (ab75596) at 1/1000 dilution

      Lane 1 : E. coli cell lysate (Gyrase A, whole protein) at 0.1 µg
      Lane 2 : E. coli cell lysate (Gyrase A, whole protein) at 0.5 µg
      Lane 3 : E. coli cell lysate (Gyrase A, whole protein) at 1 µg
      Lane 4 : E. coli cell lysate (Gyrase B, whole protein) at 0.1 µg
      Lane 5 : E. coli cell lysate (Gyrase B, whole protein) at 0.5 µg
      Lane 6 : E. coli cell lysate (Gyrase B, whole protein) at 1 µg
      Lane 7 : E. coli cell lysate (Gyrase B, B43 domain) at 0.1 µg
      Lane 8 : E. coli cell lysate (Gyrase A, A59 domain) at 0.1 µg

      Secondary
      goat anti rabbit Igs at 1/5000 dilution

      Observed band size : 90 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.

    References for Anti-DNA Gyrase B antibody (ab75596)

    ab75596 has not yet been referenced specifically in any publications.

    Product Wall



    This polyclonal antibody ab75596 it will recognise all gyrase/fragments whole protein or separate domains.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"