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DNA methylation is a covalent modification of the cytosine ring at the 5' position of a CpG dinucleotide which leads to epigenetic inactivation of genes when found in 5'-CpG-3'dinucleotides within promoters or in the first exon of genes. It is well demonstrated that DNA methylation plays an important role in the regulation of gene expression, tumorigenesis, and other genetic and epigenetic diseases.
Most methods for DNA methylation detection require a prior bisulfite-based DNA modification. Bisulfite-based DNA modification is used to discrimate between cytosine and methylated cytosine, in which DNA is treated with bisulfite salt to convert cytosine residues to uracil in a single-stranded DNA, while methylated cytosine remains the same.
DNA purification & modification Kit (ab117124) allows the user to isolate and modify DNA efficiently. The entire procedure can be completed within 2 hours and 20 minutes is suitable for MS-PCR, real time MS-PCR, methylation sequencing, and pyrosequencing, as well as methylation microarray.
Not sure if this is the right kit for you? Check out our bisulfite modification guide for more information.
|1X TE Buffer||1 x 16ml|
|DNA Binding Buffer||1 x 14ml|
|DNA Denature Solution||1 x 0.25ml|
|DNA Digestion Powder||1 vial|
|DNA Digestion Solution||1 x 1ml|
|DNA Elution Buffer||1 x 1ml|
|DNA Isolation Buffer||1 x 22ml|
|DNA Isolation Enhancer||1 x 0.1ml|
|DNA Modifier Powder||4 vials|
|DNA Modifier Solution||1 x 5ml|
|DNA Washing Buffer||1 x 3ml|
|F-Collection Tube||80 units|
|F-Spin Column||80 units|
Our Abpromise guarantee covers the use of ab117124 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab117124 has not yet been referenced specifically in any publications.