DNA Isolation & Bisulfite Conversion Kit (ab117124)

Overview

  • Product nameDNA Isolation & Bisulfite Conversion Kit
    See all DNA modification kits
  • Tests
    1 x 40 test
  • Sample type
    Serum, Plasma, Adherent cells, Suspension cells
  • Assay time
    2h 20m
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: all Mammals
  • Product overview

    DNA methylation is a covalent modification of the cytosine ring at the 5' position of a CpG dinucleotide which leads to epigenetic inactivation of genes when found in 5'-CpG-3'dinucleotides within promoters or in the first exon of genes. It is well demonstrated that DNA methylation plays an important role in the regulation of gene expression, tumorigenesis, and other genetic and epigenetic diseases.

    Most methods for DNA methylation detection require a prior bisulfite-based DNA modification. Bisulfite-based DNA modification is used to discrimate between cytosine and methylated cytosine, in which DNA is treated with bisulfite salt to convert cytosine residues to uracil in a single-stranded DNA, while methylated cytosine remains the same.
    DNA purification & modification Kit (ab117124) allows the user to isolate and modify DNA efficiently. The entire procedure can be completed within 2 hours and 20 minutes is suitable for MS-PCR, real time MS-PCR, methylation sequencing, and pyrosequencing, as well as methylation microarray.

    Not sure if this is the right kit for you? Check out our bisulfite modification guide for more information.

  • Tested applicationsSuitable for: Functional Studiesmore details

Properties

    Applications

    Our Abpromise guarantee covers the use of ab117124 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent concentration.

    Protocols

    References for DNA Isolation & Bisulfite Conversion Kit (ab117124)

    ab117124 has not yet been referenced specifically in any publications.

    Product Wall

    25 ul serum or 50 ul of plasma is fine for using with this kit. The EBM3 buffer can be directly added into the serum/plasma sample. 25 ul of serum would be fine for obtaining modified DNA. However we are not sure if the modified DNA can be obtained fro...

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    Thank you for contacting us.

    Unfortunately, we do not carry DNA purification columns, but we do carry a kit:

    EpiSeeker DNA purification & modification Kit (ab117124) [ http://www.abcam.com/EpiSeeker-DNA-purification--modificati...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"