Anti-DNA Ligase I antibody [10G12] (ab125095)

Overview

  • Product nameAnti-DNA Ligase I antibody [10G12]
    See all DNA Ligase I primary antibodies
  • Description
    Mouse monoclonal [10G12] to DNA Ligase I
  • Tested applicationsSuitable for: Sandwich ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 810-919 of Human DNA Ligase I with Propietary tag (NP_000225).

  • Positive control
    • HeLa nuclear extract; Human Thymus tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Constituent: 99% PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • Clone number10G12
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab125095 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.
WB 1/500 - 1/1000. Predicted molecular weight: 102 kDa.
IHC-P Use a concentration of 5 µg/ml.

Target

  • FunctionDNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.
  • Sequence similaritiesBelongs to the ATP-dependent DNA ligase family.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DNA ligase 1 antibody
    • DNA ligase I antibody
    • DNLI1_HUMAN antibody
    • LIG 1 antibody
    • lig1 antibody
    • Ligase I DNA ATP dependent antibody
    • MGC117397 antibody
    • MGC130025 antibody
    • Polydeoxyribonucleotide synthase [ATP] 1 antibody
    see all

Anti-DNA Ligase I antibody [10G12] images

  • Anti-DNA Ligase I antibody [10G12] (ab125095) at 1/500 dilution + HeLa nuclear extract

    Predicted band size : 102 kDa
  • ab125095, at 5 µg/ml, staining DNA Ligase I in Formalin-fixed, Paraffin-embedded Human thymus tissue by Immunohistochemistry.
  • Using ab125095 as a capture antibody, the detection limit for recombinant proprietary tagged DNA Ligase I is approximately 0.3ng/ml.

References for Anti-DNA Ligase I antibody [10G12] (ab125095)

ab125095 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab125095.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"