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- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferpH: 7.20
Constituent: 99% PBS
Concentration information loading...
- PurityProtein A purified
- Clone number10G12
- Light chain typekappa
- Research areas
- Compatible Secondaries
- Positive Controls
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use at an assay dependent concentration.
||1/500 - 1/1000. Predicted molecular weight: 102 kDa.
||Use a concentration of 5 µg/ml.
- FunctionDNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.
- Sequence similaritiesBelongs to the ATP-dependent DNA ligase family.
- Cellular localizationNucleus.
Information by UniProt
- DNA ligase 1 antibody
- DNA ligase I antibody
- DNLI1_HUMAN antibody
Anti-DNA Ligase I antibody [10G12] images
Western blot - Anti-DNA Ligase I antibody [10G12] (ab125095)
Anti-DNA Ligase I antibody [10G12] (ab125095) at 1/500 dilution + HeLa nuclear extract
Predicted band size : 102 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA Ligase I antibody [10G12] (ab125095)
ab125095, at 5 µg/ml, staining DNA Ligase I in Formalin-fixed, Paraffin-embedded Human thymus tissue by Immunohistochemistry.
Sandwich ELISA - Anti-DNA Ligase I antibody [10G12] (ab125095)
Using ab125095 as a capture antibody, the detection limit for recombinant proprietary tagged DNA Ligase I is approximately 0.3ng/ml.
References for Anti-DNA Ligase I antibody [10G12] (ab125095)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"