Overview

  • Product nameAnti-DNA Ligase I antibody [1A9]
    See all DNA Ligase I primary antibodies
  • Description
    Mouse monoclonal [1A9] to DNA Ligase I
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native protein (purified) (Cow).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: None
    Constituents: PBS
  • Concentration information loading...
  • PurityIgG fraction
  • ClonalityMonoclonal
  • Clone number1A9
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab18691 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 101 kDa.
ELISA Use at an assay dependent dilution.

Target

  • FunctionDNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.
  • Sequence similaritiesBelongs to the ATP-dependent DNA ligase family.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DNA ligase 1 antibody
    • DNA ligase I antibody
    • DNLI1_HUMAN antibody
    • LIG 1 antibody
    • lig1 antibody
    • Ligase I DNA ATP dependent antibody
    • MGC117397 antibody
    • MGC130025 antibody
    • Polydeoxyribonucleotide synthase [ATP] 1 antibody
    see all

References for Anti-DNA Ligase I antibody [1A9] (ab18691)

This product has been referenced in:
  • Prigent C  et al. Aberrant DNA repair and DNA replication due to an inherited enzymatic defect in human DNA ligase I. Mol Cell Biol 14:310-7 (1994). Read more (PubMed: 8264597) »

See 1 Publication for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Sample Human Cell (HeLa cells)
Specification HeLa cells
Permeabilization Yes - 0.2% Triton-X100
Fixative Paraformaldehyde
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Submitted Aug 01 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"