Overview

  • Product name
    Anti-DNA Ligase I antibody [1A9]
    See all DNA Ligase I primary antibodies
  • Description
    Mouse monoclonal [1A9] to DNA Ligase I
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native protein (purified) (Cow).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • Purity
    IgG fraction
  • Clonality
    Monoclonal
  • Clone number
    1A9
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab18691 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 101 kDa.
ELISA Use at an assay dependent dilution.

Target

  • Function
    DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.
  • Sequence similarities
    Belongs to the ATP-dependent DNA ligase family.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DNA ligase 1 antibody
    • DNA ligase I antibody
    • DNLI1_HUMAN antibody
    • LIG 1 antibody
    • lig1 antibody
    • Ligase I DNA ATP dependent antibody
    • MGC117397 antibody
    • MGC130025 antibody
    • Polydeoxyribonucleotide synthase [ATP] 1 antibody
    see all

References

This product has been referenced in:
  • Prigent C  et al. Aberrant DNA repair and DNA replication due to an inherited enzymatic defect in human DNA ligase I. Mol Cell Biol 14:310-7 (1994). Read more (PubMed: 8264597) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Sample
Human Cell (HeLa cells)
Specification
HeLa cells
Permeabilization
Yes - 0.2% Triton-X100
Fixative
Paraformaldehyde
Username

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Verified customer

Submitted Aug 01 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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