Overview

  • Product nameAnti-DNA Ligase I antibody
    See all DNA Ligase I primary antibodies
  • Description
    Rabbit polyclonal to DNA Ligase I
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Caenorhabditis elegans, Zebrafish
  • Immunogen

    A synthetic peptide corresponding to a region of Human DNA Ligase I (NP_000225)

  • Positive control
    • HepG2 cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab76232 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 102 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1:62500.

Target

Anti-DNA Ligase I antibody images

  • Anti-DNA Ligase I antibody (ab76232) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 102 kDa
    Observed band size : 85 kDa (why is the actual band size different from the predicted?)

References for Anti-DNA Ligase I antibody (ab76232)

This product has been referenced in:
  • Lokanga RA  et al. Heterozygosity for a hypomorphic Polß mutation reduces the expansion frequency in a mouse model of the Fragile X-related disorders. PLoS Genet 11:e1005181 (2015). WB ; Mouse . Read more (PubMed: 25886163) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab76232.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"