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Recombinant human DNA Ligase III protein encoding amino acids 1-862 purified from baculovirus-infected Sf-9 cells.
Our Abpromise guarantee covers the use of ab587 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 103 kDa.|
|Flow Cyt||Use 1µg for 106 cells.|
ab587 staining cultured human HeLa cells by ICC/IF. Cells were PFA fixed and permeabilized in 0.5% Triton X100 prior to blocking in 5% BSA for 1 hour at 20°C. The primary antibody was diluted 1/200 and incubated with the sample for 1 hour at 20°C. A Cy3® conjugated donkey anti-mouse antibody diluted was used as the secondary.