Anti-DNA Ligase III/LIG3 antibody (ab96576)
Key features and details
- Rabbit polyclonal to DNA Ligase III/LIG3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DNA Ligase III/LIG3 antibody
See all DNA Ligase III/LIG3 primary antibodies -
Description
Rabbit polyclonal to DNA Ligase III/LIG3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human DNA Ligase III/LIG3 aa 765-999 (internal sequence).
Database link: NP_039269 -
Positive control
- WB: A431, HeLa, HepG2, MOLT4, Raji cells IHC-P: H441 Xenograft
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab96576 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 113 kDa.
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IHC-P |
1/100 - 1/500. Antigen retrieval is not essential but may optimise staining.
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ICC/IF | (1) |
1/100 - 1/200.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 113 kDa. |
IHC-P
1/100 - 1/500. Antigen retrieval is not essential but may optimise staining. |
ICC/IF
1/100 - 1/200. |
Target
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Function
Interacts with DNA-repair protein XRCC1 and can correct defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. -
Tissue specificity
Testis, thymus, prostate and heart. -
Sequence similarities
Belongs to the ATP-dependent DNA ligase family.
Contains 1 BRCT domain.
Contains 1 PARP-type zinc finger. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 3980 Human
- Omim: 600940 Human
- SwissProt: P49916 Human
- Unigene: 100299 Human
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Alternative names
- D11Wsu78e antibody
- DNA ligase antibody
- DNA ligase 3 antibody
see all
Images
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Anti-DNA Ligase III/LIG3 antibody (ab96576) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg
Predicted band size: 113 kDa
7.5% SDS PAGE -
Immunofluorescence analysis of Ligase III in paraformaldehyde fixed A431, using ab96576 at a 1/200 dilution.
Lower image (2): merged with DNA probe. -
Immunohistochemical analysis of Ligase III in paraffin embedded H441 Xenograft, using ab96576 at a 1/100 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab96576 has been referenced in 1 publication.
- Rother MB et al. CHD7 and 53BP1 regulate distinct pathways for the re-ligation of DNA double-strand breaks. Nat Commun 11:5775 (2020). PubMed: 33188175