Anti-DNA Ligase III antibody (ab97404)


  • Product name
    Anti-DNA Ligase III antibody
    See all DNA Ligase III primary antibodies
  • Description
    Rabbit polyclonal to DNA Ligase III
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment containing a sequence corresponding to a region within amino acids 116-403 of Human DNA Ligase III (NP_039269).

  • Positive control
    • HepG2 whole cell lysate; A549 xenograft; 293T, A431, H1299, HeLa, Molt-4 and Raji cell lysates.


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 20% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab97404 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 113 kDa.
IHC-P 1/100 - 1/250.


  • Function
    Interacts with DNA-repair protein XRCC1 and can correct defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents.
  • Tissue specificity
    Testis, thymus, prostate and heart.
  • Sequence similarities
    Belongs to the ATP-dependent DNA ligase family.
    Contains 1 BRCT domain.
    Contains 1 PARP-type zinc finger.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • D11Wsu78e antibody
    • DNA ligase antibody
    • DNA ligase 3 antibody
    • DNA ligase III antibody
    • DNLI3_HUMAN antibody
    • LIG 3 antibody
    • LIG2 antibody
    • LIG3 antibody
    • Ligase II, DNA, ATP dependent antibody
    • Ligase III DNA ATP dependent antibody
    • MGC78176 antibody
    • OTTMUSP00000000875 antibody
    • Polydeoxyribonucleotide synthase [ATP] 3 antibody
    • Polydeoxyribonucleotide synthase [ATP] antibody
    • Polydeoxyribonucleotide synthase antibody
    • RP23-316C11.6 antibody
    see all

Anti-DNA Ligase III antibody images

  • Anti-DNA Ligase III antibody (ab97404) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size : 113 kDa

    7.5% SDS-PAGE


    Secondary antibody - anti-rabbit HRP (ab6721)

  • ab97404, at 1/100 dilution, staining DNA Ligase III in paraffin-embedded A549 xenograft by Immunohistochemistry.

References for Anti-DNA Ligase III antibody (ab97404)

ab97404 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting Abcam regarding ab97404.

I am sorry that you have been experiencing difficulties with this antibody in WB. I have reviewed the protocol information you provided and I would like to make some suggestions that may...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Western blot
Mouse Tissue lysate - whole (Brain, Heart, Lung and Kidney)
Loading amount
40 µg
Brain, Heart, Lung and Kidney
Gel Running Conditions
Reduced Denaturing (4-15% gradient)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 16 2012

Thank you for your email.

I've just added the discount code toour internal notes of your review, so it is now active and can be redeemed for a free antibody.

Please let me know if there is anything else that we can do for you. Have ...

Read More

Thank you for your reply.

An account was automatically generated for your email addressin order for us to correspond with you. You can change your password and access this account by going to "sign in" onthe http://www.abcam.comwebp...

Read More

Thank you for contacting us and for keeping us updated about the situation. I am sorry to hear that the suggestions from my colleague did not help improve the results.

At this time we have only tested ab97404 with human lysates, and though we...

Read More


Sign up