Overview

  • Product nameAnti-DNA Ligase IV antibody
    See all DNA Ligase IV primary antibodies
  • Description
    Rabbit polyclonal to DNA Ligase IV
  • Tested applicationsSuitable for: IHC-P, IP, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    A region within a synthetic peptide: IADIEHIEKD MKHQSFYIET KLDGERMQMH KDGDVYKYFS RNGYNYTDQF, corresponding to internal sequence amino acids 253-302 of DNA Ligase IV

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab26039 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 4 - 8 µg/ml.
IP Use at an assay dependent concentration. PubMed: 18451142
WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 109 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionEfficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.
  • Tissue specificityTestis, thymus, prostate and heart.
  • Involvement in diseaseLIG4 syndrome
    Severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-negative/NK-cell-positive with sensitivity to ionizing radiation
  • Sequence similaritiesBelongs to the ATP-dependent DNA ligase family.
    Contains 2 BRCT domains.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DNA ligase IV ATP dependent antibody
    • DNA joinase antibody
    • DNA ligase 4 antibody
    • DNA ligase IV antibody
    • DNA repair enzyme antibody
    • DNLI4_HUMAN antibody
    • LIG 4 antibody
    • LIG4 antibody
    • LIG4S antibody
    • Ligase IV antibody
    • Ligase IV DNA ATP dependent antibody
    • Polydeoxyribonucleotide synthase [ATP] 4 antibody
    • Polydeoxyribonucleotide synthase 4 antibody
    • Polydeoxyribonucleotide synthase antibody
    • Polynucleotide ligase antibody
    • Sealase antibody
    see all

Anti-DNA Ligase IV antibody images

  • Anti-DNA Ligase IV antibody (ab26039) at 1.25 µg/ml + HepG2 cell lysate

    Predicted band size : 109 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas tissue labelling DNA Ligase IV with ab26039 at a concentration of 4-8µg/ml. Cells with positive label (as indicated by the arrows) are epithelial cells of the pancreatic acinus. Magnification 400X.

References for Anti-DNA Ligase IV antibody (ab26039)

This product has been referenced in:
  • Rasmussen RD  et al. Enhanced efficacy of combined HDAC and PARP targeting in glioblastoma. Mol Oncol N/A:N/A (2016). WB ; Human . Read more (PubMed: 26794465) »
  • Morgenroth A  et al. Hedgehog signaling sensitizes glioma stem cells to endogenous nano-irradiation. Oncotarget 5:5483-93 (2014). Flow Cyt, ICC/IF ; Human . Read more (PubMed: 24978848) »

See all 7 Publications for this product

Product Wall

Thank you for contacting us. Your credit note ID is ****. I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice o...

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Thank you for contacting us and for providing these details.

After review of your protocol I would like to offer to you either a free of charge replace of this product or another DNA Ligase IV antibody such ashttp://www.abcam.com/DNA-Ligase-...

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Thank you for contacting us. I am sorry for the confusion with the last email. I mis-transcribed the abcam product number. This product is covered by our Abpromise guarantee.If you could send me the orginal Abcam confirmation number for purchase of ...

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Thank you for contacting us. The data which I have seen for NUCB1, from SwissProthttp://www.uniprot.org/uniprot/Q02818the NUCB1 antibody datasheet from similar products offered by other companies and our own datasheet for ab26039 all point to a pree...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - nuclear (Vascular Smooth Muscle Cell)
Loading amount 25 µg
Specification Vascular Smooth Muscle Cell
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Andrew Cobb

Verified customer

Submitted Mar 20 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (293T)
Loading amount 100 µg
Specification 293T
Treatment NCS 200ug/ul
Gel Running Conditions Non-reduced Denaturing (8%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mrs. Maayan Salton

Verified customer

Submitted May 28 2010

Thank you for getting back in touch with me with your order details. I have arranged for a credit note to be raised by our accounts department against order numbers 126743 and 114423 for two vials of ab17988. They will be in touch with your purchas...

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Thank you for your enquiry. In Charlotte's absence I am dealing with her enquiries. I am sorry to hear that you have been having difficulties with these antibodies. I have been reading through your technical questionaires and I appreciate the de...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"