Overview

  • Product name
    Anti-DNA Ligase IV antibody
    See all DNA Ligase IV primary antibodies
  • Description
    Rabbit polyclonal to DNA Ligase IV
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, IP, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    A region within a synthetic peptide: IADIEHIEKD MKHQSFYIET KLDGERMQMH KDGDVYKYFS RNGYNYTDQF, corresponding to internal sequence amino acids 253-302 of DNA Ligase IV

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab26039 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 4 - 8 µg/ml.
IP Use at an assay dependent concentration. PubMed: 18451142
WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 109 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.
  • Tissue specificity
    Testis, thymus, prostate and heart.
  • Involvement in disease
    LIG4 syndrome
    Severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-negative/NK-cell-positive with sensitivity to ionizing radiation
  • Sequence similarities
    Belongs to the ATP-dependent DNA ligase family.
    Contains 2 BRCT domains.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • DNA ligase IV ATP dependent antibody
    • DNA joinase antibody
    • DNA ligase 4 antibody
    • DNA ligase IV antibody
    • DNA repair enzyme antibody
    • DNLI4_HUMAN antibody
    • LIG 4 antibody
    • LIG4 antibody
    • LIG4S antibody
    • Ligase IV antibody
    • Ligase IV DNA ATP dependent antibody
    • Polydeoxyribonucleotide synthase [ATP] 4 antibody
    • Polydeoxyribonucleotide synthase 4 antibody
    • Polydeoxyribonucleotide synthase antibody
    • Polynucleotide ligase antibody
    • Sealase antibody
    see all

Images

  • Western blot - Anti-DNA Ligase IV antibody (ab26039)
    Western blot - Anti-DNA Ligase IV antibody (ab26039)
    Anti-DNA Ligase IV antibody (ab26039) at 1.25 µg/ml + HepG2 cell lysate

    Predicted band size: 109 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA Ligase IV antibody (ab26039)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA Ligase IV antibody (ab26039)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas tissue labelling DNA Ligase IV with ab26039 at a concentration of 4-8µg/ml. Cells with positive label (as indicated by the arrows) are epithelial cells of the pancreatic acinus. Magnification 400X.

References

This product has been referenced in:
  • Qiu Z  et al. Generation of Gross Chromosomal Rearrangements by a Single Engineered DNA Double Strand Break. Sci Rep 7:43156 (2017). Read more (PubMed: 28225067) »
  • Bermudez-Hernandez K  et al. A Method for Quantifying Molecular Interactions Using Stochastic Modelling and Super-Resolution Microscopy. Sci Rep 7:14882 (2017). Read more (PubMed: 29093506) »

See all 10 Publications for this product

Publishing research using ab26039? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Submit a question

6 Q&A

10
Votes: Highest First

Thank you so mcuh for your email and reply.  Actually, this job has been done by another antibody from different company.  If possible, please arrange a refund to us.  Thank you so much for your great help.  We silll love Abcam antibody and we will use...

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Thank you for contacting us. Your credit note ID is ****. I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice o...

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I attached detailed experimental method here for your reference.  It is a quite straightforward method and protocol which has been used b us for a quite long time. Thank you much for your help and suport!   Best wishes!

Thank you for contacting us and for providing these details.

After review of your protocol I would like to offer to you either a free of charge replace of this product or another DNA Ligase IV antibody such ashttp://www.abcam.com/DNA-Ligase-...

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http://www.abcam.com/DNA-Ligase-IV-antibody-ab26039.html here is the link for the data sheet of ab26039.

Thank you for contacting us. I am sorry for the confusion with the last email. I mis-transcribed the abcam product number. This product is covered by our Abpromise guarantee.If you could send me the orginal Abcam confirmation number for purchase of ...

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Dear Sir:



last time, we reported an technical issue about the abtibody 26039. We found that the detect band (in our result is about 70KD) which is obious different from prodicted size (109KD) mentioned in abcam manual. As we thou...

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Thank you for contacting us. The data which I have seen for NUCB1, from SwissProthttp://www.uniprot.org/uniprot/Q02818the NUCB1 antibody datasheet from similar products offered by other companies and our own datasheet for ab26039 all point to a pree...

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The purchase from the university of Maryland on 07/02/06, order number 126743 by Jay Hunter is correct. And in the absence of detection of Artemis and DNA ligase IV using ab18319 and ab26039, I ordered other antibodies for Artemis and DNA-Ligase IV a...

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Thank you for getting back in touch with me with your order details. I have arranged for a credit note to be raised by our accounts department against order numbers 126743 and 114423 for two vials of ab17988. They will be in touch with your purchas...

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Dear abcam! I hope it works this time.

Thank you for your enquiry. In Charlotte's absence I am dealing with her enquiries. I am sorry to hear that you have been having difficulties with these antibodies. I have been reading through your technical questionaires and I appreciate the de...

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