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Synthetic peptide corresponding to Human DNA PKcs (C terminal). Immunogen is in the region of aa 4075 and the c terminus.
Database link: P78527
Our Abpromise guarantee covers the use of ab70250 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 469 kDa (predicted molecular weight: 469 kDa).|
|IP||Use at an assay dependent concentration.
Use at 2 to 5 µg/mg lysate.
|ICC/IF||Use at an assay dependent concentration.|
ab70250 staining DNA PKCs in T98G Human brain glioblastoma by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, pH 7.4 for 5 minutes at room temperature and blocked with 5% BSA for 10 minutes at room temperature. Samples were incubated with primary antibody (1/200 in PBS) for 1 hour. A CF568-conjugated Donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.
ab70250 has not yet been referenced specifically in any publications.