Anti-DNA polymerase alpha antibody (ab57296)

Overview

  • Product nameAnti-DNA polymerase alpha antibody
    See all DNA polymerase alpha primary antibodies
  • Description
    Mouse monoclonal to DNA polymerase alpha
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: QFSRTGPLCP ACMKATLQPE YSDKSLYTQL CFYRYIFDAE CALEKLTTDH EKDKLKKQFF TPKVLQDYRK LKNTAEQFLS RSGYSEVNLS KLFAGCAVKS , corresponding to amino acids 1363-1463 of Human DNA polymerase alpha

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab57296 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1-5 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionPlays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes.
    • Sequence similaritiesBelongs to the DNA polymerase type-B family.
    • Post-translational
      modifications
      A 165 kDa form is probably produced by proteolytic cleavage at Lys-124.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • DKFZp686K1672 antibody
      • DNA polymerase alpha 1 catalytic subunit antibody
      • DNA polymerase alpha catalytic subunit antibody
      • DNA polymerase alpha catalytic subunit p180 antibody
      • DNA polymerase alpha p180 subunit antibody
      • DPOLA_HUMAN antibody
      • OTTHUMP00000023089 antibody
      • OTTHUMP00000023090 antibody
      • P180 antibody
      • p180 subunit antibody
      • POLA antibody
      • POLA1 antibody
      • Polymerase (DNA directed), alpha 1, catalytic subunit antibody
      • Polymerase (DNA-directed), alpha (70kD) antibody
      see all

    Anti-DNA polymerase alpha antibody images



    • Predicted band size : 166 kDa
      DNA polymerase alpha antibody (ab57296) at 1ug/lane + HeLa cell lysate at 25ug/lane.

    References for Anti-DNA polymerase alpha antibody (ab57296)

    ab57296 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"