Anti-DNA polymerase alpha antibody (ab65009)

Overview

  • Product nameAnti-DNA polymerase alpha antibody
    See all DNA polymerase alpha primary antibodies
  • Description
    Rabbit polyclonal to DNA polymerase alpha
  • SpecificityThis antibody detects endogenous levels of total DNA polymerase alpha protein.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide derived from N-terminal of human DNA polymerase alpha

  • Positive control
    • Extracts from Jurkat, 293 and HepG2 cells

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab65009 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 169 kDa (predicted molecular weight: 169 kDa).
ELISA 1/20000.

Target

  • FunctionPlays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes.
  • Sequence similaritiesBelongs to the DNA polymerase type-B family.
  • Post-translational
    modifications
    A 165 kDa form is probably produced by proteolytic cleavage at Lys-124.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp686K1672 antibody
    • DNA polymerase alpha 1 catalytic subunit antibody
    • DNA polymerase alpha catalytic subunit antibody
    • DNA polymerase alpha catalytic subunit p180 antibody
    • DNA polymerase alpha p180 subunit antibody
    • DPOLA_HUMAN antibody
    • OTTHUMP00000023089 antibody
    • OTTHUMP00000023090 antibody
    • P180 antibody
    • p180 subunit antibody
    • POLA antibody
    • POLA1 antibody
    • Polymerase (DNA directed), alpha 1, catalytic subunit antibody
    • Polymerase (DNA-directed), alpha (70kD) antibody
    see all

Anti-DNA polymerase alpha antibody images

  • All lanes : Anti-DNA polymerase alpha antibody (ab65009) at 1/500 dilution

    Lane 1 : Extracts from Jurkat cells
    Lane 2 : Extracts from 293 cells
    Lane 3 : Extracts from HepG2 cells
    Lane 4 : Extracts from HepG2 cells with immunizing peptide

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 169 kDa
    Observed band size : 169 kDa

References for Anti-DNA polymerase alpha antibody (ab65009)

ab65009 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your patience.
For ab57296, the sequence homology of the immunogen with mouse is 83% - which is good.
The lab let me know that for ab65009, the amino acid range of the immunogen is within residues 100-300 of human DNA Polymerase ...

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I am checking with the lab about the homology of the immunogen sequence with mouse. The overall homology between human and mouse is 88%, but some regions align better than others.
I will let you know when I hear back.
What applications are...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"