The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 37 kDa).
Use at an assay dependent concentration.
FunctionRepair polymerase. Conducts "gap-filling" DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity.
Sequence similaritiesBelongs to the DNA polymerase type-X family.
DomainResidues 239-252 form a flexible loop which appears to affect the polymerase fidelity.
Post-translational modificationsMethylation by PRMT6 stimulates the polymerase activity by enhancing DNA binding and processivity.