Overview

  • Product nameAnti-DNA Polymerase beta antibody
    See all DNA Polymerase beta primary antibodies
  • Description
    Rabbit polyclonal to DNA Polymerase beta
  • SpecificityThis antibody detects endogenous levels of total DNA polymerase beta.
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthesized peptide derived from human DNA Polymerase beta.

  • Positive control
    • IHC-P: Human breast carcinoma tissue. WB: Extracts from NIH/3T3 cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab53059 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 37 kDa).
ELISA 1/10000.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionRepair polymerase. Conducts "gap-filling" DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity.
  • Sequence similaritiesBelongs to the DNA polymerase type-X family.
  • DomainResidues 239-252 form a flexible loop which appears to affect the polymerase fidelity.
  • Post-translational
    modifications
    Methylation by PRMT6 stimulates the polymerase activity by enhancing DNA binding and processivity.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DNA directed DNA polymerase beta antibody
    • DNA pol beta antibody
    • DNA polymerase beta antibody
    • DNA polymerase beta subunit antibody
    • DPOLB_HUMAN antibody
    • MGC125976 antibody
    • Pol B antibody
    • Pol beta antibody
    • POLB antibody
    • Polymerase (DNA directed) beta antibody
    see all

Anti-DNA Polymerase beta antibody images

  • All lanes : Anti-DNA Polymerase beta antibody (ab53059) at 1/500 dilution

    Lane 1 : Extracts from NIH/3T3 cells
    Lane 2 : Extracts from NIH/3T3 cells treated with peptide


    Predicted band size : 37 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of DNA Polymerase beta in paraffin embedded human breast carcinoma tissue using ab53059 at a 1/50 dilution.
    Left image: untreated.
    Right image: treated with peptide.

References for Anti-DNA Polymerase beta antibody (ab53059)

This product has been referenced in:
  • Yoshizawa K  et al. Gastrointestinal hyperplasia with altered expression of DNA polymerase beta. PLoS One 4:e6493 (2009). IHC-P ; Human . Read more (PubMed: 19654874) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"