The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIHC-P: Ready to use for 60 min at room temperature .
Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionRepair polymerase. Conducts "gap-filling" DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity.
Sequence similaritiesBelongs to the DNA polymerase type-X family.
DomainResidues 239-252 form a flexible loop which appears to affect the polymerase fidelity.
Post-translational modificationsMethylation by PRMT6 stimulates the polymerase activity by enhancing DNA binding and processivity.