The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 38 kDa. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
1/2000 - 1/5000.
Repair polymerase. Conducts "gap-filling" DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity.
Belongs to the DNA polymerase type-X family.
Residues 239-252 form a flexible loop which appears to affect the polymerase fidelity.
Methylation by PRMT6 stimulates the polymerase activity by enhancing DNA binding and processivity.