Anti-DNA Polymerase delta, catalytic subunit antibody (ab168827)

Overview

  • Product name
    Anti-DNA Polymerase delta, catalytic subunit antibody
    See all DNA Polymerase delta, catalytic subunit primary antibodies
  • Description
    Rabbit polyclonal to DNA Polymerase delta, catalytic subunit
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Horse, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan, Bat
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 50-100 of Human DNA Polymerase delta, catalytic subunit (NP_002682.2).

  • Positive control
    • HeLa, 293T, Jurkat, Mouse TCMK-1 and Mouse NIH3T3 whole cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7 to 8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab168827 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 124 kDa.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Possesses two enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Required with its accessory proteins (proliferating cell nuclear antigen (PCNA) and replication factor C (RFC) or activator 1) for leading strand synthesis. Also involved in completing Okazaki fragments initiated by the DNA polymerase alpha/primase complex.
    • Sequence similarities
      Belongs to the DNA polymerase type-B family.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Polymerase (DNA directed) delta 1 catalytic subunit antibody
      • CDC2 antibody
      • CDC2 homolog antibody
      • CRCS10 antibody
      • DNA directed DNA polymerase delta 1 antibody
      • DNA directed polymerase delta 1 antibody
      • DNA pol delta 1 antibody
      • DNA polymerase delta catalytic subunit antibody
      • DNA polymerase subunit delta p125 antibody
      • DPOD1_HUMAN antibody
      • MDPL antibody
      • POLD antibody
      • POLD 1 antibody
      • POLD1 antibody
      • Polymerase (DNA directed) delta 1 catalytic subunit 125kDa antibody
      • Polymerase (DNA) delta 1 catalytic subunit antibody
      • Polymerase DNA directed delta 1 catalytic subunit 125kD antibody
      • polymerase, DNA, delta antibody
      see all

    Images

    • All lanes : Anti-DNA Polymerase delta, catalytic subunit antibody (ab168827) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate
      Lane 2 : 293T whole cell lysate
      Lane 3 : Jurkat whole cell lysate
      Lane 4 : TCMK-1 whole cell lysate
      Lane 5 : NIH3T3 whole cell lysate

      Lysates/proteins at 50 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 124 kDa


      Exposure time: 10 seconds

    References

    ab168827 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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