Anti-DNA Polymerase delta, catalytic subunit antibody (ab96796)

Overview

  • Product name
    Anti-DNA Polymerase delta, catalytic subunit antibody
    See all DNA Polymerase delta, catalytic subunit primary antibodies
  • Description
    Rabbit polyclonal to DNA Polymerase delta, catalytic subunit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant protein fragment corresponding to a region within amino acids 685 and 1041 of Human DNA Polymerase delta, catalytic subunit (NP_002682).

  • Positive control
    • MOLT4, 293T, HeLa, HepG2 or Raji cell lines.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96796 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 124 kDa.
ICC/IF 1/100 - 1/200.

Target

  • Function
    Possesses two enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Required with its accessory proteins (proliferating cell nuclear antigen (PCNA) and replication factor C (RFC) or activator 1) for leading strand synthesis. Also involved in completing Okazaki fragments initiated by the DNA polymerase alpha/primase complex.
  • Sequence similarities
    Belongs to the DNA polymerase type-B family.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Polymerase (DNA directed) delta 1 catalytic subunit antibody
    • CDC2 antibody
    • CDC2 homolog antibody
    • CRCS10 antibody
    • DNA directed DNA polymerase delta 1 antibody
    • DNA directed polymerase delta 1 antibody
    • DNA pol delta 1 antibody
    • DNA polymerase delta catalytic subunit antibody
    • DNA polymerase subunit delta p125 antibody
    • DPOD1_HUMAN antibody
    • MDPL antibody
    • POLD antibody
    • POLD 1 antibody
    • POLD1 antibody
    • Polymerase (DNA directed) delta 1 catalytic subunit 125kDa antibody
    • Polymerase (DNA) delta 1 catalytic subunit antibody
    • Polymerase DNA directed delta 1 catalytic subunit 125kD antibody
    • polymerase, DNA, delta antibody
    see all

Images

  • Anti-DNA Polymerase delta, catalytic subunit antibody (ab96796) at 1/3000 dilution + MOLT4 whole cell lysate at 30 µg

    Predicted band size : 124 kDa
  • Top: ab96796 at 1/200 dilution staining paraformaldehyde-fixed HeLa cells by Immunofluorescence.
    Bottom: image merged with DNA probe.

References

ab96796 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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