Anti-POLD2 antibody - C-terminal (ab187710)
Key features and details
- Rabbit polyclonal to POLD2 - C-terminal
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-POLD2 antibody - C-terminal
See all POLD2 primary antibodies -
Description
Rabbit polyclonal to POLD2 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rabbit, Chimpanzee, Rhesus monkey, Orangutan -
Immunogen
Synthetic peptide within Human POLD2 aa 400 to the C-terminus (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P49005 -
Positive control
- HeLa, 293T and Jurkat whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab187710 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/1000 - 1/5000. Predicted molecular weight: 51 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 51 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
Target
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Function
The function of the small subunit is not yet clear. -
Sequence similarities
Belongs to the DNA polymerase delta/II small subunit family. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 472654 Chimpanzee
- Entrez Gene: 5425 Human
- Entrez Gene: 100460756 Orangutan
- Entrez Gene: 100358263 Rabbit
- Entrez Gene: 700100 Rhesus monkey
- Omim: 600815 Human
- SwissProt: P49005 Human
- Unigene: 306791 Human
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Alternative names
- DNA polymerase delta subunit 2 antibody
- DNA polymerase delta subunit p50 antibody
- DNA polymerase subunit delta 2 antibody
see all
Images
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All lanes : Anti-POLD2 antibody - C-terminal (ab187710) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 51 kDa
Exposure time: 3 minutes -
Immunoprecipitation of POLD2 from 293T whole cell lysate (1 mg per lane), using ab187710 at 6 µg (lane 1) or an IgG control (lane 2). For western blot of immunoprecipitated protein, ab187710 was used at 1 µg/ml.
Detection: ECL with an exposure time of 30 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab187710 has not yet been referenced specifically in any publications.