This antibody gave a positive signal in the following Human tissue lysates: Heart; Brain; Placenta; Testis; Skeletal muscle.
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pH: 7.40 Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Chromatin accessibility complex 17 kDa protein antibody
DNA Polymerase Epsilon p17 Subunit antibody
DNA polymerase epsilon subunit 3 antibody
DNA polymerase epsilon subunit p17 antibody
DNA polymerase II subunit 3 antibody
Histone fold protein CHRAC17 antibody
HuCHRAC 17 antibody
p17 subunit antibody
POL E3 antibody
POLE 3 antibody
Polymerase (DNA directed) epsilon 3 antibody
YBL 1 antibody
Western blot - Anti-DNA Polymerase epsilon antibody (ab105454)
All lanes : Anti-DNA Polymerase epsilon antibody (ab105454) at 1 µg/ml
Lane 1 : Human heart tissue lysate - total protein (ab29431) Lane 2 : Human brain tissue lysate - total protein (ab29466) Lane 3 : Human placenta tissue lysate - total protein (ab29745) Lane 4 : Human testis tissue lysate - total protein (ab30257) Lane 5 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lysates/proteins at 10 µg per lane.
Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 16 kDa Observed band size : 16 kDa Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.
ICC/IF image of ab105454 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab105454, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% formaldehyde fixed (10 min) HeLa cells at 1µg/ml.