Anti-DNA Polymerase epsilon antibody (ab84532)

Overview

  • Product nameAnti-DNA Polymerase epsilon antibody
    See all DNA Polymerase epsilon primary antibodies
  • Description
    Rabbit polyclonal to DNA Polymerase epsilon
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Guinea pig, Cow, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan, Bat
  • Immunogen

    A region between residue 97 and 147 of human DNA polymerase epsilon (NP_059139.2)

  • Positive control
    • Human Anaplastic Thyroid Carcinoma, Bladder Cell Carcinoma, Breast Carcinoma, Colon Carcinoma, Metastatic Lymph Node, Ovarian Carcinoma, Prostate Carcinoma, Skin Basal Cell Carcinoma, Small Cell Lung Cancer, Stomach Adenocarcinoma or Testicular Seminoma tissue. Mouse Teratoma tissue.
  • General notesAntibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. Shelf Life: 1 year from date of receipt

Properties

Applications

Our Abpromise guarantee covers the use of ab84532 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notesIHC-P: 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining. In some cases, the antibody may be diluted further than indicated.

    Likely to work with frozen sections

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionForms a complex with DNA polymerase epsilon subunit CHRAC1 and binds naked DNA, which is then incorporated into chromatin, aided by the nucleosome-remodeling activity of ISWI/SNF2H and ACF1.
    • Tissue specificityExpressed in all tissues tested, including, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Arsenic transactivated protein antibody
      • Arsenic-transactivated protein antibody
      • ASTP antibody
      • CHARAC 17 antibody
      • CHARAC17 antibody
      • CHRAC 17 antibody
      • CHRAC-17 antibody
      • CHRAC17 antibody
      • Chromatin Accessibility Complex 17 antibody
      • Chromatin accessibility complex 17 kDa protein antibody
      • DNA Polymerase Epsilon p17 Subunit antibody
      • DNA polymerase epsilon subunit 3 antibody
      • DNA polymerase epsilon subunit p17 antibody
      • DNA polymerase II subunit 3 antibody
      • DPOE3_HUMAN antibody
      • Histone fold protein CHRAC17 antibody
      • HuCHRAC 17 antibody
      • HuCHRAC17 antibody
      • p17 antibody
      • p17 subunit antibody
      • POL E3 antibody
      • POLE 3 antibody
      • POLE3 antibody
      • Polymerase (DNA directed) epsilon 3 antibody
      • YBL 1 antibody
      • YBL1 antibody
      see all

    Anti-DNA Polymerase epsilon antibody images

    • ab84532, at a 1:100 dilution, staining human DNA polymerase epsilon in breast carcinoma, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue. Detection: DAB staining using an Immunohistochemistry Accessory Kit
    • ab84532, at a 1:500 dilution, staining human DNA polyermase epsilon in colon carcinoma, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue. Detection: DAB staining using an Immunohistochemistry Accessory Kit
    • ab84532, at a 1:100 dilution, staining mouse DNA polymerase epsilon in teratoma, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue. Detection: DAB staining using an Immunohistochemistry Accessory Kit

    References for Anti-DNA Polymerase epsilon antibody (ab84532)

    ab84532 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"