Anti-DNA Polymerase epsilon antibody (ab88521)

Overview

  • Product name
    Anti-DNA Polymerase epsilon antibody
    See all DNA Polymerase epsilon primary antibodies
  • Description
    Mouse polyclonal to DNA Polymerase epsilon
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length Human protein (AAH03166.1)

  • Positive control
    • Human liver tissue lysate. DNA Polymerase epsilon transfected 293T cell lysate. HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab88521 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 17 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function
    Forms a complex with DNA polymerase epsilon subunit CHRAC1 and binds naked DNA, which is then incorporated into chromatin, aided by the nucleosome-remodeling activity of ISWI/SNF2H and ACF1.
  • Tissue specificity
    Expressed in all tissues tested, including, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Arsenic transactivated protein antibody
    • Arsenic-transactivated protein antibody
    • ASTP antibody
    • CHARAC 17 antibody
    • CHARAC17 antibody
    • CHRAC 17 antibody
    • CHRAC-17 antibody
    • CHRAC17 antibody
    • Chromatin Accessibility Complex 17 antibody
    • Chromatin accessibility complex 17 kDa protein antibody
    • DNA Polymerase Epsilon p17 Subunit antibody
    • DNA polymerase epsilon subunit 3 antibody
    • DNA polymerase epsilon subunit p17 antibody
    • DNA polymerase II subunit 3 antibody
    • DPOE3_HUMAN antibody
    • Histone fold protein CHRAC17 antibody
    • HuCHRAC 17 antibody
    • HuCHRAC17 antibody
    • p17 antibody
    • p17 subunit antibody
    • POL E3 antibody
    • POLE 3 antibody
    • POLE3 antibody
    • Polymerase (DNA directed) epsilon 3 antibody
    • YBL 1 antibody
    • YBL1 antibody
    see all

Images

  • Anti-DNA Polymerase epsilon antibody (ab88521) at 1 µg/ml + Human liver tissue lysate at 50 µg

    Predicted band size : 17 kDa
    Observed band size : 20 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-DNA Polymerase epsilon antibody (ab88521) at 1 µg/ml

    Lane 1 : DNA Polymerase epsilon transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 17 kDa
    Observed band size : 17 kDa
  • ab88521, at 10 µg/ml, staining DNA Polymerase epsilon in HeLa cells by Immunofluorescence.

References

ab88521 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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