DNA Polymerase epsilon overexpression 293T lysate (whole cell) (ab94038)

Overview

  • Product name
    DNA Polymerase epsilon overexpression 293T lysate (whole cell)
    See all DNA Polymerase epsilon lysates
  • General notes
    ab94038 is a 293T cell transfected lysate in which Human DNA Polymerase epsilon has been transiently over-expressed using a pCMV-DNA Polymerase epsilon plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
  • Tested applications
    Suitable for: WBmore details

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 6.80
    Constituent: 100% 1x Sample Buffer
  • Concentration information loading...
  • Purity
    Whole Cell Lysate
  • Research areas
  • Background
    Function: Forms a complex with DNA polymerase epsilon subunit CHRAC1 and binds naked DNA, which is then incorporated into chromatin, aided by the nucleosome-remodeling activity of ISWI/SNF2H and ACF1. Tissue specificity: Expressed in all tissues tested, including, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

Associated products

Applications

Our Abpromise guarantee covers the use of ab94038 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

Images

  • ab94038 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-DNA Polymerase epsilon antibody (ab88521) at 1/500 dilution

    Lane 1 : DNA Polymerase epsilon 293T Transfected Lysate - Positive Control (ab94038)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References

ab94038 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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