Anti-POLG antibody (ab97661)
Key features and details
- Rabbit polyclonal to POLG
- Suitable for: ICC/IF, IP, IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-POLG antibody
See all POLG primary antibodies -
Description
Rabbit polyclonal to POLG -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide corresponding to Human POLG aa 779-1201.
Database link: NP_002684 -
Positive control
- WB: MCF7, MDA-MB-231 cell and rat testis tissue lysate; IHC-P: Human skeletal muscle tissue; IF: HeLa cells; IP: MCF7 cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97661 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/100 - 1/1000.
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IP |
1/100 - 1/500.
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IHC-P |
1/100 - 1/1000.
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WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 140 kDa.
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Notes |
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ICC/IF
1/100 - 1/1000. |
IP
1/100 - 1/500. |
IHC-P
1/100 - 1/1000. |
WB
1/500 - 1/3000. Predicted molecular weight: 140 kDa. |
Target
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Function
Involved in the replication of mitochondrial DNA. -
Involvement in disease
Defects in POLG are the cause of progressive external ophthalmoplegia with mitochondrial DNA deletions autosomal dominant type 1 (PEOA1) [MIM:157640]. Progressive external ophthalmoplegia is characterized by progressive weakness of ocular muscles and levator muscle of the upper eyelid. In a minority of cases, it is associated with skeletal myopathy, which predominantly involves axial or proximal muscles and which causes abnormal fatigability and even permanent muscle weakness. Ragged-red fibers and atrophy are found on muscle biopsy. A large proportion of chronic ophthalmoplegias are associated with other symptoms, leading to a multisystemic pattern of this disease. Additional symptoms are variable, and may include cataracts, hearing loss, sensory axonal neuropathy, ataxia, depression, hypogonadism, and parkinsonism.
Defects in POLG are a cause of progressive external ophthalmoplegia with mitochondrial DNA deletions autosomal recessive (PEOB) [MIM:258450]. PEOB is a severe form of progressive external ophthalmoplegia. It is clinically more heterogeneous than the autosomal dominant forms. Can be more severe.
Defects in POLG are a cause of sensory ataxic neuropathy dysarthria and ophthalmoparesis (SANDO) [MIM:607459]. SANDO is a clinically heterogeneous systemic disorder with variable features resulting from mitochondrial dysfunction. It shares phenotypic characteristics with autosomal recessive progressive external ophthalmoplegia and mitochondrial neurogastrointestinal encephalopathy syndrome. The clinical triad of symptoms consists of sensory ataxic, neuropathy, dysarthria, and ophthalmoparesis.
Defects in POLG are a cause of Alpers-Huttenlocher syndrome (AHS) [MIM:203700]; also called Alpers diffuse degeneration of cerebral gray matter with hepatic cirrhosis. AHS is an autosomal recessive hepatocerebral syndrome. The typical course of AHS includes severe developmental delay, intractable seizures, liver failure, and death in childhood. Refractory seizures, cortical blindness, progressive liver dysfunction, and acute liver failure after exposure to valproic acid are considered diagnostic features. The neuropathological hallmarks of AHS are neuronal loss, spongiform degeneration, and astrocytosis of the visual cortex. Liver biopsy results show steatosis, often progressing to cirrhosis.
Defects in POLG are a cause of mitochondrial neurogastrointestinal encephalopathy syndrome (MNGIE) [MIM:603041]; also known as myoneurogastrointestinal encephalomyopathy. MNGIE is an autosomal recessive disease associated with multiple deletions of skeletal muscle mitochondrial DNA (MtDNA). It is clinically characterized by onset between the second and fifth decades of life, ptosis, progressive external ophthalmoplegia, gastrointestinal dysmotility (often pseudoobstruction), diffuse leukoencephalopathy, thin body habitus, peripheral neuropathy, and myopathy.
Defects in POLG are a cause of Leigh syndrome (LS) [MIM:256000]. LS is a severe neurological disorder characterized by bilaterally symmetrical necrotic lesions in subcortical brain regions. -
Sequence similarities
Belongs to the DNA polymerase type-A family. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 5428 Human
- Entrez Gene: 85472 Rat
- Omim: 174763 Human
- SwissProt: P54098 Human
- SwissProt: Q9QYV8 Rat
- Unigene: 706868 Human
- Unigene: 19327 Rat
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Alternative names
- DNA directed DNA polymerase gamma antibody
- DNA polymerase subunit gamma 1 antibody
- DNA polymerase subunit gamma-1 antibody
see all
Images
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All lanes : Anti-POLG antibody (ab97661) at 1/1000 dilution
Lane 1 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 2 : MDA-MB-231 (human breast adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 140 kDa5% SDS-PAGE
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Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue staining DNA polymerase gamma with ab97661 at 10 μg/ml.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min. -
Immunofluorescence analysis of methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
labeling DNA polymerase gamma with ab97661 at 1/200 dilution. Costained with Hoechst 33342. -
Immunoprecipitation of DNA polymerase gamma protein from MCF7 (human breast adenocarcinoma cell line) whole cell lysate using 5 μg ab97661.
IP Sample:
1- MCF7 cells
2- Control IgG
3- ab97661
Western blot analysis was performed using ab97661. followed by anti-Rabbit IgG antibody. -
Anti-POLG antibody (ab97661) at 1/1000 dilution + Rat testis tissue lysate at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 140 kDa5% SDS-PAGE
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab97661 has not yet been referenced specifically in any publications.