Anti-DNA Polymerase iota antibody (ab123331)

Overview

  • Product name
    Anti-DNA Polymerase iota antibody
    See all DNA Polymerase iota primary antibodies
  • Description
    Rabbit polyclonal to DNA Polymerase iota
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within C terminal amino acids 647-676 of Human DNA Polymerase iota (NP_009126.2).

  • Positive control
    • HL60 cell line lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab123331 was purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab123331 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 83 kDa.

Target

  • Function
    Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity.
  • Tissue specificity
    Ubiquitous. Highly expressed in testis.
  • Sequence similarities
    Belongs to the DNA polymerase type-Y family.
    Contains 1 umuC domain.
  • Domain
    The catalytic core consists of fingers, palm and thumb subdomains, but the fingers and thumb subdomains are much smaller than in high-fidelity polymerases; residues from five sequence motifs of the Y-family cluster around an active site cleft that can accommodate DNA and nucleotide substrates with relaxed geometric constraints, with consequently higher rates of misincorporation and low processivity.
  • Cellular localization
    Nucleus. Accumulates at replication forks after DNA damage.
  • Information by UniProt
  • Database links
  • Alternative names
    • DNA polymerase iota antibody
    • Eta 2 antibody
    • Eta2 antibody
    • POLI antibody
    • POLI_HUMAN antibody
    • Polymerase (DNA directed) iota antibody
    • RAD 30B antibody
    • RAD30 homolog B antibody
    • RAD30, S. cerevisiae, homolog of antibody
    • RAD30B antibody
    • RAD3OB antibody
    see all

Images

  • Anti-DNA Polymerase iota antibody (ab123331) at 1/100 dilution + HL60 cell line lysates at 35 µg

    Predicted band size : 83 kDa

References

ab123331 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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