Anti-DNA Polymerase iota antibody - N-terminal (ab104319)

Overview

  • Product nameAnti-DNA Polymerase iota antibody - N-terminal
    See all DNA Polymerase iota primary antibodies
  • Description
    Rabbit polyclonal to DNA Polymerase iota - N-terminal
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide designed within residues: PVVERLGFDE NFVDLTEMVE KRLQQLQSDE LSAVTVSGHV YNNQSINLLD, corresponding to N terminal amino acids 118 - 167 of Human DNA Polymerase iota (NP_009126).

  • Positive control
    • Fetal lung lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: None
    Constituents: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab104319 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 80 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionError-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity.
  • Tissue specificityUbiquitous. Highly expressed in testis.
  • Sequence similaritiesBelongs to the DNA polymerase type-Y family.
    Contains 1 umuC domain.
  • DomainThe catalytic core consists of fingers, palm and thumb subdomains, but the fingers and thumb subdomains are much smaller than in high-fidelity polymerases; residues from five sequence motifs of the Y-family cluster around an active site cleft that can accommodate DNA and nucleotide substrates with relaxed geometric constraints, with consequently higher rates of misincorporation and low processivity.
  • Cellular localizationNucleus. Accumulates at replication forks after DNA damage.
  • Information by UniProt
  • Database links
  • Alternative names
    • DNA polymerase iota antibody
    • Eta 2 antibody
    • Eta2 antibody
    • POLI antibody
    • POLI_HUMAN antibody
    • Polymerase (DNA directed) iota antibody
    • RAD 30B antibody
    • RAD30 homolog B antibody
    • RAD30, S. cerevisiae, homolog of antibody
    • RAD30B antibody
    • RAD3OB antibody
    see all

Anti-DNA Polymerase iota antibody - N-terminal images

  • Anti-DNA Polymerase iota antibody - N-terminal (ab104319) at 1 µg/ml + Human fetal lung lysate at 10 µg

    Predicted band size : 80 kDa

References for Anti-DNA Polymerase iota antibody - N-terminal (ab104319)

ab104319 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"