Anti-DNA Polymerase Kappa/POLK antibody (ab115625)

Overview

  • Product name
    Anti-DNA Polymerase Kappa/POLK antibody
    See all DNA Polymerase Kappa/POLK primary antibodies
  • Description
    Goat polyclonal to DNA Polymerase Kappa/POLK
  • Tested applications
    Suitable for: ELISA, WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide:

    PVNQPKESSRSTGS

    corresponding to amino acids 817-830 of Human DNA Polymerase Kappa/POLK, conjugated to KLH.

  • Positive control
    • Human prostate tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab115625 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/2000 - 1/10000.
WB 1/500 - 1/3000. Predicted molecular weight: 99 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent dilution.

Target

  • Function
    DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. Lacks 3'-5' proofreading exonuclease activity. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity.
  • Tissue specificity
    Detected at low levels in testis, spleen, prostate and ovary. Detected at very low levels in kidney, colon, brain, heart, liver, lung, placenta, pancreas and peripheral blood leukocytes.
  • Sequence similarities
    Belongs to the DNA polymerase type-Y family.
    Contains 2 UBZ-type zinc fingers.
    Contains 1 umuC domain.
  • Domain
    The catalytic core consists of fingers, palm and thumb subdomains, but the fingers and thumb subdomains are much smaller than in high-fidelity polymerases; residues from five sequence motifs of the Y-family cluster around an active site cleft that can accommodate DNA and nucleotide substrates with relaxed geometric constraints, with consequently higher rates of misincorporation and low processivity.
  • Cellular localization
    Nucleus. Detected throughout the nucleus and at replication foci.
  • Information by UniProt
  • Database links
  • Alternative names
    • DinB homolog 1 (E. coli) antibody
    • DINB protein antibody
    • DINB1 antibody
    • DINP antibody
    • DNA damage inducible protein b antibody
    • DNA polymerase kappa antibody
    • Polk antibody
    • POLK_HUMAN antibody
    • POLQ antibody
    • Polymerase (DNA directed) kappa antibody
    • polymerase, DNA, kappa antibody
    see all

Images

  • ab115625, at 5 µg/ml, staining DNA Polymerase Kappa/POLK in formalin fixed, paraffin embedded Human prostate tissue by Immunohistochemistry. Following primary incubation slides were incubated with biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

References

ab115625 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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