Anti-DNA Polymerase Kappa/POLK antibody (ab86076)
Key features and details
- Rabbit polyclonal to DNA Polymerase Kappa/POLK
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DNA Polymerase Kappa/POLK antibody
See all DNA Polymerase Kappa/POLK primary antibodies -
Description
Rabbit polyclonal to DNA Polymerase Kappa/POLK -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan -
Immunogen
Synthetic peptide corresponding to Human DNA Polymerase Kappa/POLK aa 650-750.
Database link: Q9UBT6 -
Positive control
- WB: HeLa or 293T whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab86076 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 99 kDa.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 99 kDa. |
Target
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Function
DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. Lacks 3'-5' proofreading exonuclease activity. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity. -
Tissue specificity
Detected at low levels in testis, spleen, prostate and ovary. Detected at very low levels in kidney, colon, brain, heart, liver, lung, placenta, pancreas and peripheral blood leukocytes. -
Sequence similarities
Belongs to the DNA polymerase type-Y family.
Contains 2 UBZ-type zinc fingers.
Contains 1 umuC domain. -
Domain
The catalytic core consists of fingers, palm and thumb subdomains, but the fingers and thumb subdomains are much smaller than in high-fidelity polymerases; residues from five sequence motifs of the Y-family cluster around an active site cleft that can accommodate DNA and nucleotide substrates with relaxed geometric constraints, with consequently higher rates of misincorporation and low processivity. -
Cellular localization
Nucleus. Detected throughout the nucleus and at replication foci. - Information by UniProt
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Database links
- Entrez Gene: 51426 Human
- Omim: 605650 Human
- SwissProt: Q9UBT6 Human
- Unigene: 726312 Human
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Alternative names
- DinB homolog 1 (E. coli) antibody
- DINB protein antibody
- DINB1 antibody
see all
Images
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All lanes : Anti-DNA Polymerase Kappa/POLK antibody (ab86076) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 99 kDa
Detection: chemiluminescence with exposure time of 3 minutes
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab86076 has not yet been referenced specifically in any publications.