The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 63 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/12500.
FunctionRepair polymerase. Involved in base excision repair (BER) responsible for repair of lesions that give rise to abasic (AP) sites in DNA. Has both DNA polymerase and terminal transferase activities. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity.
Tissue specificityExpressed in a number of tissues. Abundant in testis.
Sequence similaritiesBelongs to the DNA polymerase type-X family. Contains 1 BRCT domain.
Post-translational modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.