Anti-DNA Polymerase lambda antibody (ab84750)

Overview

  • Product name
    Anti-DNA Polymerase lambda antibody
    See all DNA Polymerase lambda primary antibodies
  • Description
    Rabbit polyclonal to DNA Polymerase lambda
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Guinea pig, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region between residues 525 and 575 of human DNA Polymerase lambda (NP_037406.1)

  • Positive control
    • Human controls: Anaplastic Thyroid Carcinoma, Breast Carcinoma, Linitis Plastica Stomach Cancer, Ovarian Carcinoma, Pancreatic Islet Cell Tumor, Prostate Carcinoma, Stomach Adenocarcinoma, Testicular Seminoma tissues. Mouse controls: Teratoma tissue.
  • General notes
    Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. Centrifuge microtube to remove product from lid.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab84750 was affinity purified using an epitope specific to DNA Polymerase lambda immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab84750 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notes
    IHC-P: 1/100 - 1/500.
    Epitope exposure with citrate buffer will enhance staining.

    Likely to work with Frozen Sections.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Repair polymerase. Involved in base excision repair (BER) responsible for repair of lesions that give rise to abasic (AP) sites in DNA. Has both DNA polymerase and terminal transferase activities. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity.
    • Tissue specificity
      Expressed in a number of tissues. Abundant in testis.
    • Sequence similarities
      Belongs to the DNA polymerase type-X family.
      Contains 1 BRCT domain.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • BETA N antibody
      • BETAN antibody
      • DNA directed DNA polymerase lambda antibody
      • DNA polymerase beta 2 antibody
      • DNA polymerase beta-2 antibody
      • DNA polymerase kappa antibody
      • DNA polymerase kappa DNA polymerase beta N antibody
      • DNA polymerase lambda antibody
      • DNA polymerase lamda2 antibody
      • DPOLL_HUMAN antibody
      • EC 2.7.7.7,EC 4.2.99. antibody
      • FLJ46002 antibody
      • OTTHUMP00000020321 antibody
      • OTTHUMP00000020323 antibody
      • OTTHUMP00000059179 antibody
      • Pol beta2 antibody
      • POL KAPPA antibody
      • Pol Lambda antibody
      • POLKAPPA antibody
      • POLL antibody
      • Polymerase DNA directed lambda antibody
      see all

    Anti-DNA Polymerase lambda antibody images

    • ab84750 at 1/250 dilution staining DNA Polymerase lambda in human prostate nodular hypertrophy by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.
    • ab84750 at 1/250 dilution staining DNA Polymerase lambda in mouse teratoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.

    References for Anti-DNA Polymerase lambda antibody (ab84750)

    ab84750 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab84750.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up