Anti-DNA Polymerase theta antibody (ab77151)

Overview

  • Product nameAnti-DNA Polymerase theta antibody
    See all DNA Polymerase theta primary antibodies
  • Description
    Mouse monoclonal to DNA Polymerase theta
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Recombinant Fragment
    Predicted to work with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 1609-1717 of Human DNA Polymerase theta

  • Positive control
    • Recombinant tagged human DNA Polymerase theta fragment (the immunogen).

Properties

Applications

Our Abpromise guarantee covers the use of ab77151 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 198 kDa. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
ELISA Use at an assay dependent dilution. Detection limit for recombinant tagged DNA Polymerase theta is approximately 1 ng/ml when used as a capture antibody.

Target

  • FunctionHas a DNA polymerase activity on nicked double-stranded DNA and on a singly primed DNA template. The enzyme activity is resistant to aphidicolin, and inhibited by dideoxynucleotides. Exhibites a single-stranded DNA-dependent ATPase activity. Could be involved in the repair of interstrand cross-links.
  • Tissue specificityHighly expressed in testis.
  • Sequence similaritiesBelongs to the DNA polymerase type-A family.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp781A0112 antibody
    • DNA polymerase eta antibody
    • DNA polymerase theta antibody
    • DPOLQ_HUMAN antibody
    • POLH antibody
    • POLQ antibody
    • Polymerase (DNA directed) theta antibody
    • PRO0327 antibody
    see all

Anti-DNA Polymerase theta antibody images

  • Anti-DNA Polymerase theta antibody (ab77151) at 5 µg/ml + recombinant tagged human DNA Polymerase theta fragment at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 198 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)

References for Anti-DNA Polymerase theta antibody (ab77151)

ab77151 has not yet been referenced specifically in any publications.

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