The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 198 kDa. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Use at an assay dependent dilution. Detection limit for recombinant tagged DNA Polymerase theta is approximately 1 ng/ml when used as a capture antibody.
FunctionHas a DNA polymerase activity on nicked double-stranded DNA and on a singly primed DNA template. The enzyme activity is resistant to aphidicolin, and inhibited by dideoxynucleotides. Exhibites a single-stranded DNA-dependent ATPase activity. Could be involved in the repair of interstrand cross-links.
Tissue specificityHighly expressed in testis.
Sequence similaritiesBelongs to the DNA polymerase type-A family. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.