Overview

  • Product name
  • Description
    Rabbit polyclonal to DNAH1
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: RIVKVMDDYQ VMDEFLYNLS SDDFNDKWIA SNWPSKILGQ IELVQQQHVE DEEKFRKIQI MDQNNFQEKL EGLQLVVAGF S, corresponding to amino acids 884-964 of Human DNAH1

  • Positive control
    • Human nasopharynx tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122367 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Force generating protein of respiratory cilia. Produces force towards the minus ends of microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP. Involved in sperm motility; implicated in sperm flagellar assembly.
  • Tissue specificity
    Expressed primarily in trachea and testis, 2 tissues containing axonemal structures. Also expressed in brain.
  • Sequence similarities
    Belongs to the dynein heavy chain family.
  • Domain
    Dynein heavy chains probably consist of an N-terminal stem (which binds cargo and interacts with other dynein components), and the head or motor domain. The motor contains six tandemly-linked AAA domains in the head, which form a ring. A stalk-like structure (formed by two of the coiled coil domains) protrudes between AAA 4 and AAA 5 and terminates in a microtubule-binding site. A seventh domain may also contribute to this ring; it is not clear whether the N-terminus or the C-terminus forms this extra domain. There are four well-conserved and two non-conserved ATPase sites, one per AAA domain. Probably only one of these (within AAA 1) actually hydrolyzes ATP, the others may serve a regulatory function.
  • Cellular localization
    Cytoplasm > cytoskeleton > cilium axoneme.
  • Information by UniProt
  • Database links
  • Alternative names
    • axonemal antibody
    • Axonemal beta dynein heavy chain 1 antibody
    • Ciliary dynein heavy chain 1 antibody
    • DNAH1 antibody
    • DNAHC1 antibody
    • DYH1_HUMAN antibody
    • Dynein heavy chain 1 antibody
    • hDHC7 antibody
    • Heat shock regulated protein 1 antibody
    • HL 11 antibody
    • HL11 antibody
    • HSRF-1 antibody
    • XLHSRF-1 antibody
    see all

Anti-DNAH1 antibody images

  • Immunofluorescent staining of Human cell line U-251 MG shows positivity in nuclear membrane and cytoplasm. Recommended concentration of ab122367 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122367, at 1/500 dilution, staining DNAH1 in paraffin-embedded Human nasopharynx tissue by immunohistochemistry.

References for Anti-DNAH1 antibody (ab122367)

ab122367 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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