Overview

  • Product name
    Anti-DNAH12 antibody
  • Description
    Rabbit polyclonal to DNAH12
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    C terminal amino acids 2993-3044 (IHGLYLDGAR WDRESGLLAE QYPKLLFDLM PIIWIKPTQK SRIIKSDAYV CP) of the Human DNAH12 protein (Q6ZR08).

  • Positive control
    • Human colon tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122394 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

  • Function
    Force generating protein of respiratory cilia. Produces force towards the minus ends of microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP. Involved in sperm motility; implicated in sperm flagellar assembly.
  • Sequence similarities
    Belongs to the dynein heavy chain family.
  • Domain
    Dynein heavy chains probably consist of an N-terminal stem (which binds cargo and interacts with other dynein components), and the head or motor domain. The motor contains six tandemly-linked AAA domains in the head, which form a ring. A stalk-like structure (formed by two of the coiled coil domains) protrudes between AAA 4 and AAA 5 and terminates in a microtubule-binding site. A seventh domain may also contribute to this ring; it is not clear whether the N-terminus or the C-terminus forms this extra domain. There are four well-conserved and two non-conserved ATPase sites, one per AAA domain. Probably only one of these (within AAA 1) actually hydrolyzes ATP, the others may serve a regulatory function.
  • Cellular localization
    Cytoplasm > cytoskeleton > cilium axoneme.
  • Information by UniProt
  • Database links
  • Alternative names
    • axonemal antibody
    • Axonemal beta dynein heavy chain 12 antibody
    • Axonemal dynein heavy chain 12-like protein antibody
    • Axonemal dynein heavy chain 7-like protein antibody
    • Ciliary dynein heavy chain 12 antibody
    • DHC3 antibody
    • DLP12 antibody
    • Dnah12 antibody
    • DNAH12L antibody
    • DNAH7L antibody
    • DNAHC3 antibody
    • DNHD2 antibody
    • DYH12_HUMAN antibody
    • Dynein heavy chain 12 antibody
    • Dynein heavy chain 7-like antibody
    • Dynein heavy chain domain-containing protein 2 antibody
    • HDHC3 antibody
    • HL19 antibody
    see all

Images

  • All lanes : Anti-DNAH12 antibody (ab122394) at 1/250 dilution

    Lane 1 : RT4 cell lysate
    Lane 2 : U251 MG cell lysate
    Lane 3 : Human Plasma cell lysate
    Lane 4 : Liver cell lysate
    Lane 5 : Tonsil cell lysate
  • ab122394, at 1/50, staining DNAH12 in paraffin embedded Human colon tissue by immunohistochemistry.

References

ab122394 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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