Overview

  • Product name
    Anti-DNAH2 antibody
  • Description
    Rabbit polyclonal to DNAH2
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within N terminal amino acids 439-468 of Human DNAH2 (NP_065928.2; UniProt Q9P225 isoform 3).

  • Positive control
    • HL60 cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab123683 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab123683 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 100 kDa.

Target

  • Function
    Force generating protein of respiratory cilia. Produces force towards the minus ends of microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP. Involved in sperm motility; implicated in sperm flagellar assembly.
  • Tissue specificity
    Expressed primarily in trachea and testis, 2 tissues containing axonemal structures. Also expressed in lung.
  • Sequence similarities
    Belongs to the dynein heavy chain family.
    Contains 5 TPR repeats.
  • Domain
    Dynein heavy chains probably consist of an N-terminal stem (which binds cargo and interacts with other dynein components), and the head or motor domain. The motor contains six tandemly-linked AAA domains in the head, which form a ring. A stalk-like structure (formed by two of the coiled coil domains) protrudes between AAA 4 and AAA 5 and terminates in a microtubule-binding site. A seventh domain may also contribute to this ring; it is not clear whether the N-terminus or the C-terminus forms this extra domain. There are four well-conserved and two non-conserved ATPase sites, one per AAA domain. Probably only one of these (within AAA 1) actually hydrolyzes ATP, the others may serve a regulatory function.
  • Cellular localization
    Cytoplasm > cytoskeleton > cilium axoneme.
  • Information by UniProt
  • Database links
  • Alternative names
    • axonemal antibody
    • Axonemal beta dynein heavy chain 2 antibody
    • Ciliary dynein heavy chain 2 antibody
    • Dnah2 antibody
    • DYH2_HUMAN antibody
    • Dynein heavy chain 2 antibody
    • Dynein heavy chain domain-containing protein 3 antibody
    see all

Images

  • Anti-DNAH2 antibody (ab123683) at 1/100 dilution + HL60 cell lysate at 35 µg

    Predicted band size : 100 kDa

References

ab123683 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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